RE: IHC controls- storage?
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From: | Cynthia Favara <cfavara@niaid.nih.gov> |
To: | "histonet@pathology.swmed.edu" <HistoNet@pathology.swmed.edu>, "'Gervaip@aol.com'" <Gervaip@aol.com> |
Reply-To: | |
Date: | Fri, 30 Jul 1999 09:34:07 -0400 |
Content-Type: | text/plain |
Pearl,
Nice article in J of Clin Path97;50 422-428 suggests that slide
could be held for up to six months. I suspect each epitope is diferent and
perhaps you may want to carry out your own study of the procedures you use.
Studies I do I am careful to cut controls on the same day as specimens.
I look forward to comments from others.
Cynthia Favara
Rocky Mountain Laboratories
903 S 4th Street
Hamilton, MT 59840
ph: 406-363-9317
FAX: 406-363-9286
e-mail: cfavara@nih.gov
> ----------
> From: Gervaip@aol.com[SMTP:Gervaip@aol.com]
> Sent: Thursday, July 29, 1999 7:00 PM
> To: histonet@pathology.swmed.edu
> Subject: IHC controls- storage?
>
> Presently, IHC controls, are sectioned on a daily basis for IHC requests.
> Is
> there any reason why they cannot be sectioned in batches and stored in
> slide
> boxes for future use? Our lab did that at one time and it made life so
> much
> easier and I did not think that it affected the quality of the stain. One
>
> exception: the ER, PR and Factor 13 controls, were sectioned in small
> quantities and stored in a slide box in the refrigerator. It would be
> interesting to find out what other folks are doing.
>
> One main reason we presently do it this way is to keep the cost down...
> we
> can put several negative controls on one slide, when we have a request for
>
> several antibodies on the same tissue sample. And with the Ventana, the
> detection kit already costs $7.50 per slide or $15 for a set of one
> positve
> and one negative.
>
> Pearl Gervais
>
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