This could in fact be a problem with your microwave, and it would also
depend on the tissue type that you are using.  My experience has been the
same with any microwaving staining procedures, especially those with bone.
This could also be a result of the thickness of the tissue in question.
What type of adhesive do you use?

> ----------
> From: 	Pat Kokoszka[SMTP:koza@vgh.org]
> Sent: 	Monday, July 26, 1999 2:28 PM
> To: 	'histonet@pathology.swmed.edu'
> Subject: 	Gomori's trichrome stain
> 
> Does anyone out there have any suggestions regarding the microwave
> Gomori's Trichrome procedure?  I get my kit from Sigma and have been doing
> this particular stain for a number of years.  However, in the past year or
> so, I have been having a problem with the tissue coming off of the slide
> in the trichrome stain or the acid step.  I don't feel that my microwave
> is really the problem and I use an adhesive on my slides.  Any suggestions
> would be greatly appreciated.
> 
> Thanks, 
> Gwendolyn
>