RE: Floating Debris

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Date:Thu, 22 Jul 1999 12:49:20 -0500
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Maybe I missed an earlier answer to this question but, we have to filter
daily or "skim" the surface of our hematoxylin to prevent these floating
debris that my boss lovingly refers ro as "crap". It's much worse in the
summer months (humidity?). Hope this helps, Donna Montague

-----Original Message-----
From: []
Sent: Wednesday, July 21, 1999 9:27 PM
Subject: Floating Debris

I have talked with several other histotechs about debris on the H&E slides.

We have looked at everything we can think of, waterbaths, water, distilled 
water, adhesives, stains, stainers, reagents, etc.  The problem is debris 
floating on top of the stained sections.  It occurs often but not every 
single day.  I can go for a week or so with no debris and then it is back.  
The pathologists and histotechs are pulling their hair out at 2 institutions

here in Florida.  (The debris is not the hair!)  We have compared notes and 
use different products but have the same problem.  Any ideas, suggestions 
(hair removal techniques)?

Tonia Breckenridge
Baptist Hospital
Pensacola, FL

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