RE: Fleas'n' Flies!!

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From:Cynthia Favara <cfavara@niaid.nih.gov>
To:"histoNet@Pathology.swmed.edu" <HistoNet@Pathology.swmed.edu>, "'Bruce Abaloz'" <b.abaloz@zoology.unimelb.edu.au>
Reply-To:
Date:Tue, 27 Jul 1999 10:31:49 -0400
Content-Type:text/plain

Bruce,
	I have done serial sections on fleas and have just used my regular
processing schedule. Didn't find the need to soften the exoskeleton. If you
want more particulars let me know. The hardest thing was embedding for the
proper orientation.
Regards,
Cynthia Favara
Rocky Mountain Laboratories
903 S 4th Street
Hamilton, MT 59840
ph: 406-363-9317
FAX: 406-363-9286
e-mail: cfavara@nih.gov


> ----------
> From: 	Bruce Abaloz[SMTP:b.abaloz@zoology.unimelb.edu.au]
> Sent: 	Friday, July 23, 1999 12:55 AM
> To: 	histoNet@Pathology.swmed.edu
> Subject: 	Fleas'n' Flies!!
> 
> Dear Histonetters..here I am again with another "softening" question!! I
> have another student who wants to do serial sections thru fleas'n'flies to
> look @ the ovaries.(& obtain some wonderful X-sections of said beasts!!)
> The procedure she has,says "after fixing,transfer directly to SUPERCEDROL
> for a min.of 24 hrs.changing X3!! Does anyone know what this product
> /solution is, if it is a "softening agent"( for the exoskeleton) & where I
> can obtain it OR any method that has worked for you...Cheers,Bruce in OZ. 
> 
> --
> BRUCE ABALOZ
> HISTOLOGIST		      
> DEPARTMENT of ZOOLOGY	         *  ph:    +61 3 93446282
> The UNIVERSITY of MELBOURNE      *  fax:   +61 3 93447909 
> Parkville Victoria.3052          *  email: b.abaloz@zoology.unimelb.edu.au
> 
> AUSTRALIA.
>                     IF YOU WANT TO BE HAPPY......BE!!
>                     I DO NOT SEEK............I FIND!!
> 
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