Jamie,
	Just got a sample and will try to get to it soon. Please keep me
informed.

Cynthia Favara
Rocky Mountain Laboratories
903 S 4th Street
Hamilton, MT 59840
ph: 406-363-9317
FAX: 406-363-9286
e-mail: cfavara@nih.gov


> ----------
> From: 	cmcorp[SMTP:cmcorp@cellmarque.com]
> Sent: 	Monday, July 26, 1999 9:19 PM
> To: 	Shotsberger-Gray, Wanda
> Cc: 	HistoNet@Pathology.swmed.edu
> Subject: 	RE: Deparaffinizing/unmasking Solution
> 
> Jaime,
> 
> Some points to consider:
> 
> 1. The pressure cooker we offer costs $299. Compare to cost of specialized
> microwave ovens. Also, performance is excellent. 
> 
> 2. The timer on this pressure cooker does not begin to count down until
> the
> cooker becomes pressurized so it is very convenient to use and you get
> great run-to-run consistency. 
> 
> 3. The protocol is the only one that allows you to deparaffinize with heat
> and greatly reduces the procedural variability that can be associated with
> deparaffinization (this contributes to more consistent stains). Also, the
> procedure does not vary with the number of slides, and can be used
> effectively in conjunction with so many antibodies that your procedure
> manual can be greatly simplified.
> 
> 4. Declere requires no special handling because it is non-toxic and
> biodegradable. Not so for xylene and alcohols which are toxic, flammable,
> and are expensive to dispose of.
> 
> 5. Call me for a sample.
> 
> Regards,
> Paul Ardi
> Cell Marque Corp.
> 1-800-665-7284
> 
> At 02:54 PM 7/26/99 -0500, you wrote:
> >Jamie, We tried Declere here and got results comparable to regular old
> HIER.
> >As I am not the primary person doing immuno, I am not sure why we do not
> use
> >it routinely; if I had to guess, I would say ease of putting a rack in
> our
> >Energy Beam microwaves vs having to set up the pressure cooker, bring it
> up
> >to temp, remember to check if it is up to temp in the first placeand...
> well
> >you get the idea.  My guess is in our highly automated lab, it was just
> >easier to go with HIER.  (We have automated stainers, and our microwaves
> >have temperature probes).  If you need more specific info, just e-mail me
> >back.
> >Wanda Shotsberger
> >Harris Methodist Hospital
> >Fort Worth Texas
> > ----------
> >From: Jamie Erickson
> >To: HistoNet@Pathology.swmed.edu
> >Subject: Deparaffinizing/unmasking Solution
> >Date: Friday, July 23, 1999 10:33AM
> >
> >Hello,
> >         Wanted to know if anyone has tried this new solution from Cell
> >Marque called DeClere. It is a solution that deparaffinizes, rehydrates
> and
> >unmasked your paraffin samples in a pressure cooker. I am looking into
> >giving it a try and wanted to get some feed back.
> >
> >Thanks
> >Jamie
> >
> > 
> >
> >
> >
> >
>