RE: Deparaffinizing/unmasking Solution

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From:"Shotsberger-Gray, Wanda" <>, Jamie Erickson <>
Date:Mon, 26 Jul 1999 14:54:00 -0500

Jamie, We tried Declere here and got results comparable to regular old HIER.
As I am not the primary person doing immuno, I am not sure why we do not use
it routinely; if I had to guess, I would say ease of putting a rack in our
Energy Beam microwaves vs having to set up the pressure cooker, bring it up
to temp, remember to check if it is up to temp in the first placeand... well
you get the idea.  My guess is in our highly automated lab, it was just
easier to go with HIER.  (We have automated stainers, and our microwaves
have temperature probes).  If you need more specific info, just e-mail me
Wanda Shotsberger
Harris Methodist Hospital
Fort Worth Texas
From: Jamie Erickson
Subject: Deparaffinizing/unmasking Solution
Date: Friday, July 23, 1999 10:33AM

         Wanted to know if anyone has tried this new solution from Cell
Marque called DeClere. It is a solution that deparaffinizes, rehydrates and
unmasked your paraffin samples in a pressure cooker. I am looking into
giving it a try and wanted to get some feed back.



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