Jennifer,
I use the Crystalmount just as I would use any aqueous mounting media.  I
place it on the slide, then place a coverslip over it.  I know the
directions that come with the Crystalmount say do not coverslip, but it has
not affected anything.  Just make sure you have enough Crystalmount so when
the water evaporates, it does not leave bubbles. Good luck.

Joe Nocito, B.S., HT(ASCP)QIHC
Histology Supervisor
Christus Santa Rosa Hospitals
San Antonio, Texas 


> -----Original Message-----
> From:	MacDonald, Jennifer [SMTP:jmacdonald@sach.org]
> Sent:	Thursday, July 22, 1999 2:53 PM
> To:	'Judy Trogadis'; HistoNet@Pathology.swmed.edu; 'Nocito, Joseph'
> Subject:	RE: DAB question
> 
> Joseph:
> 
> Would you please share your procedure for the Crystalmount.  We are using
> AEC as our sole detection, and the Aquamount is semi-permanent.  Thank
> you.
> 
> Jennifer MacDonald
> 
> 
> > ----------
> > From: 	Nocito, Joseph[SMTP:joseph_nocito@srhc.iwhs.org]
> > Sent: 	Wednesday, July 21, 1999 10:00 AM
> > To: 	'Judy Trogadis'; HistoNet@Pathology.swmed.edu
> > Subject: 	RE: DAB question
> > 
> > Judy,
> > when we have to use AEC, we use Crystalmount from Fisher.  We use the
> > Crystalmount as a media and then place a coverslip over the top.  I have
> > looked back after a couple of years and the reaction was still there.
> The
> > catalog number is BM-M02 and is about $30 for 30 mLs.  Hope this helps
> > 
> > Joe Nocito, B.S., HT(ASCP)QIHC
> > Histology Supervisor
> > Christus Santa Rosa Hospitals
> > San Antonio, Texas 
> > 
> > 
> >