RE: DAB question

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From:Rebecca Hartley <rebeccahartley@earthlink.net>
To:"'HistoNet Server'" <HistoNet@Pathology.swmed.edu>
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Date:Thu, 22 Jul 1999 07:02:34 -0700
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Hi Judy -

I am the one with the recent question on this - I'm still trying to work 
something out here.  I still don't know why this is happening, and I'm 
actually glad to hear that someone else has had this problem.  I have been 
using the Vector Elite ABC and Sigma DAB tablets also.  I wouldn't suggest 
using metal-enhanced DAB as I've tried that and it is worse, if anything. 
 I'm experimenting with other mounting media and air dehydration.  I had 
never used the DAB tablets before, but it doesn't seem to make much sense 
to me that this would be the problem as there IS good staining at first. 
 I'd really appreciate it if you would post any helpful responses that you 
get, and if you work this out.  I will as well.  If you want to discuss 
exact details send me an email and we can set up a time to talk by phone. 
 Good luck!

Rebecca Hartley
Layton BioScience, Inc.
Gilroy, CA
rebeccahartley@earthlink.net




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