PMMA thin sections vs thick sections, gluing

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From:Gayle Callis <>
Date:Mon, 26 Jul 1999 08:43:58 -0600

I superglued bone slab sections 100 um thick or thicker onto plastic slides
with superglue (sure wish John's glue was around when I did this!) This was
for ground/polished sections only, which were never any thinner than 70 um
or so.  These sections were surface stained, different than a typical 
thin section stain. 

If I microtomed a thin PMMA sections, 5 to 10 micrometers thick, I would 
try the plus charge slides or use Haupts gelatin.  Super glue is a tidge
hazardous for laying a thin section down on slide, plus you should work in
a hood, not touch or breathe the stuff. I never tried this, but it is
tempting, just to see if it would work, barring the chemical hazard part.
I was never too enamored with superglue, dislike the smell and mess it
left behind.

Gayle Callis

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