When we are cutting our muscle block and subsequently staining for PAS, I have
noticed that a difference in staining intensity exists, even on the same slide
(i.e. same block, simply the next section).  Since we are using the staining
intensity as an indicator of glycogen stores (via densitometry) it is important
to ensure standardisation across all samples measured.  There is no streaking
evident, it is just that some sections come out darker than the others.

Any thoughts on this?

Thanks in advance
Tim.
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Timothy J. Fairchild B.Sc. (Hons)
PhD Candidate
Co-ordinator for Centre of Athletic Testing
Department of Human Movement and Exercise Science
Nedlands, Western Australia 6907
Telephone: (+61 8) 9380 2793
Facsimile:  (+61 8) 9380 1039
Email: timf@cyllene.uwa.edu.au
http://www.general.uwa.edu.au/~hmweb/index.htm