Fwd: RE: PMMA section glue
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From: | Linda Jenkins <jlinda@ces.clemson.edu> |
To: | histonet@Pathology.swmed.edu |
Reply-To: | |
Date: | Sun, 25 Jul 1999 18:43:02 -0400 |
Content-Type: | text/plain; charset="us-ascii" |
Dear Ashley:
Interesting question! I suspect that it might work with a little
"twiddling". First of all - how thin? If you are sectioning 4 - 5 micron
sections on an automated microtome, why couldn't you lay the section down
on a glass slide containing a drop of super-glue? Then put a piece of
saran wrap on top and roll flat. Quickly remove saran wrap and wipe off
any excess glue. Hmmm....do you do a lot of thin sectioning of PMMA? I
hope you try it - would love to know if it works. 'Course I wouldn't
recommend attaching the glass slide directly to the block as I do the
plastic slides. Plastic slides give - glass doesn't - might shatter.
Also, will have to develop a drop-and- roll technique to keep air bubbles
from forming underneath the slide. Acetone will remove the super-glue.
Xylene will not. If you try it, how 'bout writing a short article about
it for the "Hard Times" newsletter?
Thanks for starting my day with a challenge:-)
Linda
PS. Just read Gayle's comment. Hmm... sounds like she's tried it and it
doesn't work. Bummer! However, John Tarpley's comment about the different
super-glue's was quite fascinating -- will have to visit wood shop. Bet
wood dust smells a lot better than bone dust:-)
>
>>Would this method be difficult using glass slides and a microtome? We use
>>this method when transfering thick sections of PMMA to plastic slides to be
>>ground and pollished before staining. True, the sections definately stay
>>on!
>>However, I am not sure that this would be effective using thin sections on
>>glass slides.
*********************************
Linda Jenkins, HT
Clemson University
Department of Bioengineering
Clemson, SC
**********************************
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