Fwd: RE: PMMA section glue

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From:Linda Jenkins <jlinda@ces.clemson.edu>
Date:Sun, 25 Jul 1999 18:43:02 -0400
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Dear Ashley:
	Interesting question!  I suspect that it might work with a little
"twiddling".  First of all - how thin?  If you are sectioning 4 - 5 micron
sections on an automated microtome, why couldn't you lay the section down
on a glass slide containing a drop of super-glue?  Then put a piece of
saran wrap on top and roll flat.  Quickly remove saran wrap and wipe off
any excess glue.  Hmmm....do you do a lot of thin sectioning of PMMA?  I
hope you try it - would love to know if it works.  'Course I wouldn't
recommend attaching the glass slide directly to the block as I do the
plastic slides.  Plastic slides give - glass doesn't - might shatter.
Also, will have to develop a drop-and- roll technique to keep air bubbles
from forming underneath the slide. Acetone will remove the super-glue.
Xylene will not.   If you try it, how  'bout writing a short article about
it for the "Hard Times" newsletter?
		Thanks for starting my day with a challenge:-)
PS.  Just read Gayle's comment.  Hmm... sounds like she's tried it and it
doesn't work. Bummer!  However, John Tarpley's comment about the different
super-glue's  was quite fascinating -- will have to visit wood shop.  Bet
wood dust smells a lot better than bone dust:-)
>>Would this method be difficult using glass slides and a microtome?  We use
>>this method when transfering thick sections of PMMA to plastic slides to be
>>ground and pollished before staining.  True, the sections definately stay
>>However, I am not sure that this would be effective using thin sections on
>>glass slides.
Linda Jenkins, HT
Clemson University
Department of Bioengineering
Clemson, SC

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