Why is this person using a Lucifer Yellow/Streptavidin conjugate?  Lucifer Yellow 
is a great intracellular dye used successfully by neurosceientists for years, but 
it quite a wimpy fluorophore for use in other applications.  Is this person using 
the Lucifer Yellow because she doesn't have the right filter set for the more 
robust fluorphores? If so, I'd say it was time to bite the bullet and buy new 
filter sets, and extend her fluorescence capabilities.

Karen Larison in Oregon 


Date:          Thu, 15 Jul 1999 14:24:52 -0400
From:          "Michael J. Lyon, Ph.D." <lyonm@VAX.CS.HSCSYR.EDU>
Subject:       FW: Alexa 430
To:            Histonet <HistoNet@Pathology.swmed.edu>

I was asked to forward the following:

Michael J. Lyon, Ph.D.
Associate Professor Otolaryngology
315-464-7253 Voice
315-464-5572  FAX

-----Original Message-----
From:	Confocal Microscopy List [mailto:CONFOCAL@LISTSERV.ACSU.BUFFALO.EDU]
On Behalf Of Linda Bookman
Sent:	Monday, July 12, 1999 11:53 AM
To:	CONFOCAL@LISTSERV.ACSU.BUFFALO.EDU
Subject:	Alexa 430

Recently, Molecular Probes has stopped producing the LuciferYellow
conjugated to streptavidin. Their substitute product, Alexa 430 was highly
touted and was supposed to be an improvement on the Lucifer.  However, we
are finding on our initial use that not only do we have to use the Alexa at
an incredibly high 1:10 dilution to get any usable signal, but it also fades
almost immediately. We have tried mounting in both Vectashield and glycerine
with no appreciable difference in fade resistance. Does anyone know of
another vendor for the Streptavidin/Lucifer yellow conjugate, and failing
that does anyone have any suggestions for improving the signal on the Alexa
430?
Chris Jones