Bone and IHC

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From:cjohnston@notes.mdacc.tmc.edu
To:"HistoNet Server" <HistoNet@Pathology.swmed.edu>
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Date:Thu, 22 Jul 1999 12:56:29 -0500
Content-Type:text/plain; charset=us-ascii

I am in the process of try to find a source for ostopontin and ostelcalcin.  IHC
is very new to me and I am at a loss as to where to begin.  I also need some
advice on decalcification.  How important is the fixative?  Do I use 10% NBF or
some other fixative?  This work is being done on sheep ribs.  I can use all the
help I can get.

Thank you

Carol Johnston, HT
M.D. Anderson Cancer Center
1515 Holcombe Blvd. Box 62
Houston, Tx   77030
713-745-4625
cjohnston@notes.mdacc.tmc.edu





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