periodic acid and GMS
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From: | Gayle Callis <uvsgc@msu.oscs.montana.edu> |
To: | histonet@Pathology.swmed.edu |
Reply-To: | |
Date: | Thu, 1 Jul 1999 09:00:21 -0600 |
Content-Type: | |
Using periodic acid for a fungus stain can present problems. Read the
publication in june, 1999 J Histotechnology titled Inconsistent detection
of Histoplasma capsulatum with periodic acid oxidation in the Grocott
methenamine silve nitrate (GMS) fungus stain, F Carson, J Fredenburgh and
J Maxwell.
Time in the periodic acid should be extended to 1 hour at 56-60C, so as to not
miss H capsulatum. Aspergillus sp/C neoformans at 5 - 30 min/1% periodic acid
stained consistently. Beware the false negative, and use good positive tissue
controls of specific fungi you are trying to stain using periodic acid.
I applaud the authors of this article, very informative and timely!
Gayle Callis
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