paraformaldehyde fixed frozens freezer storage
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From: | Gayle Callis <uvsgc@msu.oscs.montana.edu> |
To: | histonet@Pathology.swmed.edu |
Reply-To: | |
Date: | Tue, 6 Jul 1999 10:54:10 -0600 |
Content-Type: | |
Good morning,
We do not store PFA (paraformaldehyde) fixed frozens, just proceed directly
with immunostaining. Question: for those who fix frozen sections with
this fixative, do you store PBS rinsed, air dried sections at -80C for
future IHC staining. OR do you store them in PBS until staining can
be done?
Interested in some storage protocols for PFA fixed frozens.
Many thanks,
Gayle Callis
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