paraformaldehyde fixed frozens freezer storage

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From:Gayle Callis <>
Date:Tue, 6 Jul 1999 10:54:10 -0600

Good morning,

We do not store PFA (paraformaldehyde) fixed frozens, just proceed directly
with immunostaining.  Question:  for those who fix frozen sections with
this fixative, do you store PBS rinsed, air dried sections at -80C for
future IHC staining.  OR do you store them in PBS until staining can
be done?  

Interested in some storage protocols for PFA fixed frozens.

Many thanks,
Gayle Callis

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