freezing muscle

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From:"Instrumedics, Inc." <>
To:"HistoNet Server" <>
Date:Fri, 2 Jul 1999 11:18:57 -0400
Content-Type:text/plain; charset="iso-8859-1"

It has been suggested that the CryoGel embedding medium has cryoprotectant
properties. If true It might be useful for freezing muscle

We have had success freezing muscle using the Gentle Jane method. The
freezing is very rapid
(8-10 sec.) The heat extractor  stored in LN2 is equilibrated to -196C.  In
addition the heat extractor is 3/4lb with a mirror finish providing good
thermal exchange. The combination leads to the formation of amorphous ice,
minimizing ice-crystal artifact.


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