TEM: Heat pen for flattening sections

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From:"Jaci Lett" <jmlett@cid.wustl.edu>
To:"Histonet Listserver" <Histonet@Pathology.swmed.edu>, "Microscopy Listserver" <ListServer@sparc5.MICROSCOPY.COM>
Date:Wed, 7 Jul 1999 13:15:37 -0500
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Does anyone have any opinions on using a heat pen to flatten ultrathin EM
sections?  I would very much prefer to get away from using chloroform.

Electron Microscopy Sciences has two models:  Wax Pen 1 (which uses 1 AA
battery) and Wax Pen 2 (which uses 2 AA batteries).  Their tech
representative recommended the Wax Pen 2 for flattening sections.  Is this
consistent with the preference of the majority?

I need to decide to place an order soon (the end of the fiscal year is

Thank you,

Jaclynn Lett, Research Assistant     jmlett@cid.wustl.edu

Fay and Carl Simon Center for the Biology of Hearing and Deafness
Central Institute for the Deaf
818 S. Euclid Ave.
St. Louis, MO 63110

voice  314-977-0257     fax  314-977-0030

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