Re: fix with IHC on brain

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From:"Tony Henwood" <>
To:<>, lynn_brennan@smtplink.Coh.ORG
Date:Tue, 13 Jul 1999 07:21:51 +0000
Content-Type:text/plain; charset=US-ASCII

Dear Lynn,

> What is the best fixative (you can make in the lab) using IHC and frozen
> sections on embryonic spinal cords?
> Any suggestions for the best fixative with any brain tissues?

The tried and true 10% formalin is very hard to beat. Millonigs 
formulation is good and gives quite acceptable ultrastructural 

For fixation of frozen sections, ethanol or Schoobridge's formulation 
(Formal alcohol) gives good morphology and is a good place to start 
for immunohistochemistry.

Hopes this helps,

Tony Henwood
Senior Scientist
Anatomical Pathology
Royal Prince Alfred Hospital

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