Re: destaining of special stain and restaining with ER/PR

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From:"Histomail\\" <>
To:Donna Sitrin <>
Date:Thu, 15 Jul 1999 08:53:08 +1000
Content-Type:text/plain; charset="iso-8859-1"

Dear Donna,
Methanol will get out 90% of Romanowsky dye, but will perform much better
with 0.5% HCl or other acid in it. If you don't have easy access to acids,
use about 10% vinegar in Methanol (White variety). Stain removal time is
controlled by dipping repeatedly, rinsing in Methanol then water and
checking for completeness, repeat if necessary. The whole excercise should
only take about 2-3 mins, anything remaining after this time is likely to be
permanent. If there is any residual pink colour, add one pellet of sodium
hydroxide to about 50ml methanol and retreat for 30-60 secs, then rinse in
methanol then water. (High pH solvent will remove eosin component).
Best wishes , Mike Rentsch. (Downunder)
-----Original Message-----
From: Donna Sitrin <>
To: <>
Date: Thursday, 15 July 1999 8:05
Subject: destaining of special stain and restaining with ER/PR

>I'm working on an important project that is going to require the destaining
>of an FNA smear(Wright-Giemsa).
>There may not be any acid alcohol components available, and this will need
>to be restained with a Diff Quik and possibly with an ER/PR.  Does anyone
>have any words of wisdom as far as the best method of destaining that will
>not thoroughly destroy receptor
>Thank you so much. This is a humanitarian effort.
>Donna Sitrin
>Unipath LLC, Denver

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