>
> When I stain with May-Grunwald, I notice that if I don't move quickly
> through the ethanols when I 'm dehydrating, I can loose quite a bit of
> my stain.  Is this the reason why?  I think I may adjust to using 3 x
> 100% next time.
>
Dear Catherine,

Unfortunately the MGG stain will eventually leach out in the alcohol.

Try air drying after washing and then coverslipping or
Dehydrate in acetone prior to clearing and coverslipping.

Regards,

Tony H
.
 
Tony Henwood
Senior Scientist
Anatomical Pathology
Royal Prince Alfred Hospital
Sydney, AUSTRALIA

http://www2.one.net.au/~henwood
http://www.pathsearch.com/homepages/TonyHenwood/default.html