Re: TEM: Heat pen for flattening sections
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From: | "R.Wadley" <s9803537@pop3.unsw.edu.au> |
To: | Histonet@Pathology.swmed.edu |
Reply-To: | |
Date: | Fri, 09 Jul 1999 09:07:17 +1000 |
Content-Type: | text/plain; charset="us-ascii" |
Dear Jaclynn,
I was taught to cut ultathins (on an LKB II) & flatten them with a heat
pen. The pen I used then was mains powered through a small transformer. I
think later models had a switch so that they come on only when you want the
heat.
I checked with my suppliers web site & they suggest your battery powered
pen can be linked to a battery eliminator (a transformer plugged into the
mains?). http://www.proscitech.com.au/index.htm
Heat pens are very effective on Spurrs resin. I used to section my epon
ultrathin sections onto 20% ethanol which flattened them out very nicely
without need of anything else. I didn't get the same result with Spurr's
although I didn't try very hard as I perfer not to use it.
Hope this helps.
Regards
Rob W.
At 01:15 PM 7/7/99 -0500, you wrote:
>Does anyone have any opinions on using a heat pen to flatten ultrathin EM
>sections? I would very much prefer to get away from using chloroform.
>Electron Microscopy Sciences has two models: Wax Pen 1 (which uses 1 AA
>battery) and Wax Pen 2 (which uses 2 AA batteries). Their tech
>representative recommended the Wax Pen 2 for flattening sections. Is this
>consistent with the preference of the majority?
>I need to decide to place an order soon (the end of the fiscal year is
>approaching).
>Thank you,
>Jaclynn Lett, Research Assistant jmlett@cid.wustl.edu
>Fay and Carl Simon Center for the Biology of Hearing and Deafness
>Central Institute for the Deaf
>818 S. Euclid Ave, St. Louis, MO 63110
>voice 314-977-0257 fax 314-977-0030
R. Wadley, B.App.Sc, M.L.S
Laboratory Manager
Cellular Analysis Facility
School of Microbiology & Immunology
UNSW, New South Wales, Australia, 2052
Ph (BH) +61 (2) 9385 3517
Ph (AH) +61 (2) 9555 1239
Fax +61 (2) 9385 1591
E-mail r.wadley@unsw.edu.au
www http://www.micro.unsw.edu.au/caf.html
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