Here ya go, Safranin 0 stain for cartilage


FIXATIVE: 10% NBF, PARAFFIN PROCESSING AND SECTIONS CUT AT 6 MICRONS.

NEEDED:  WEIGERT'S IRON HEMATOXYLIN

        FAST GREEN:  1 GRAM FAST GREEN IN 5000 ML OF DISTILLED WATER.

        1% ACETIC ACID:  1 ML ACETIC ACID IN 99 ML OF DISTILLED WATER

        .1% SAFRANIN O:  .1 GRAM SAFRANIN O IN 100 ML OF DISTILLED WATER.

PROCEDURE:

        1- DEPARAFFINIZE SLIDES THROUGH GRADED ALCOHOLS TO WATER.

        2-STAIN IN WORKING WEIGERT'S HEMATOXYLIN FOR 7 MINUTES

        3-WASH IN RUNNING TAP WATER FOR 10 MINUTES

        4-COUNTERSTAIN IN AQUEOUS FAST GREEN FOR 3 MINUTES

        5-RINSE AND AGITATE SLIDES IN 1% ACETIC ACID FOR 15 MINUTES

        6-STAIN IN .1% SAFRANIN O FOR 5 MINUTES

        7-DEHYDRATE SLIDES IN 95% ALCOHOL, ABSOLUTE ALCOHOL (3 CHANGES),
AND CLEAR IN 3 CHANGES OF XYLENE.

        8-MOUNT WITH APPROPRIATE MOUNTING MEDIA.

RESULTS:  NUCLEAR CHROMATIN:  BLACK
          CYTOPLASM:  GRAY-GREEN
          MUCOUS, CARTILAGE, AND MAST CELL GRANULES:  ORANGE-RED

Jim
_________________________
James E. Staruk, HT(ASCP)
Mass Histology Service
http://www.masshistology.com 

----- Original Message ----- 
From: Patrick M. Haley <pmhales@cybergap.net>
To: Histonet <HistoNet@Pathology.swmed.edu>
Sent: Tuesday, July 06, 1999 11:07 AM
Subject: Safranin O and Fast Green


> Fellow Histonetters,
>     I am looking for a procedure for a Safranin O & Fast Green Stain on
> cartilage and bone.
> Any ideas?
> Thanks for your help.
> 
> Pat Haley B.S. HTL(ASCP)
> Chief Technologist
> HistoTechNologies, inc.
> 
>