Re: Pancreatic Islet cells!

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From:"Jim Staruk" <Jstaruk@masshistology.com>
To:<Histonet@Pathology.swmed.edu>
Reply-To:
Date:Sat, 3 Jul 1999 11:32:01 -0400
Content-Type:text/plain; charset="iso-8859-1"

We perform a lot of islet work and have found the old, traditional dye
stains too unspecific for differentiation of the three main islet cells.
Insulin, glucagon and somatostatin antibody staining is our preferred
technique.  All three antibodies work on most species, no pre-treatment is
required for formalin-fixed, paraffin-embedded tissue and the specific cells
stain beautifully.

Let me know if you want our protocols.

Jim
____________________________
James E. Staruk, HT(ASCP)
Mass Histology Service
http://www.masshistology.com

----- Original Message -----
From: <jennifer.hoover@pharma.Novartis.com>
To: <Histonet@Pathology.swmed.edu>
Sent: Friday, July 02, 1999 10:18 AM
Subject: Pancreatic Islet cells!


>      Hi Histonetters!  I am seeking input for Aldehyde Fucshin staining of
islet
> cells.  Currently I am doing histological work on 10% NBF fixed paraffin
> embedded mouse kidneys in which islet cells (also mouse) have been
transplanted
> under the renal capsule.  I have consulted Sheehan's book for  Gomori's
Aldehyde
> Fucshin staining of pancreatic islet cells but have had little success.
The
> stain functions beautifully for the elastin tissue in the vasculature but
there
> is no staining of islet cells; even in normal pancreas tissue.  I know
that the
> stain needs to be prepared fresh and is good for only a few weeks. I have
also
> substituted acetaldehyde for paraldehyde in preparing the stain.  Still no
good
> staining of islets!  Any suggestions would be most greatly appreciated!
Thanks
> in advance!
>
>
> Jennifer Hoover
> Histologist
> Transplantation Biology
> Novartis Pharmaceuticals
> Summit, NJ
>
>
>




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