Re: Pancreatic Islet cells!

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From:"Jim Staruk" <>
Date:Sat, 3 Jul 1999 11:32:01 -0400
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We perform a lot of islet work and have found the old, traditional dye
stains too unspecific for differentiation of the three main islet cells.
Insulin, glucagon and somatostatin antibody staining is our preferred
technique.  All three antibodies work on most species, no pre-treatment is
required for formalin-fixed, paraffin-embedded tissue and the specific cells
stain beautifully.

Let me know if you want our protocols.

James E. Staruk, HT(ASCP)
Mass Histology Service

----- Original Message -----
From: <>
To: <>
Sent: Friday, July 02, 1999 10:18 AM
Subject: Pancreatic Islet cells!

>      Hi Histonetters!  I am seeking input for Aldehyde Fucshin staining of
> cells.  Currently I am doing histological work on 10% NBF fixed paraffin
> embedded mouse kidneys in which islet cells (also mouse) have been
> under the renal capsule.  I have consulted Sheehan's book for  Gomori's
> Fucshin staining of pancreatic islet cells but have had little success.
> stain functions beautifully for the elastin tissue in the vasculature but
> is no staining of islet cells; even in normal pancreas tissue.  I know
that the
> stain needs to be prepared fresh and is good for only a few weeks. I have
> substituted acetaldehyde for paraldehyde in preparing the stain.  Still no
> staining of islets!  Any suggestions would be most greatly appreciated!
> in advance!
> Jennifer Hoover
> Histologist
> Transplantation Biology
> Novartis Pharmaceuticals
> Summit, NJ

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