You're right, after it has ripened for 2-5 days it's fine for use, but
after7-10 days activity starts dropping off, yet it's fine for elastica for
up to 10months if kept @ 4C with a minimum airspace. Always use a known
control and pull out your previous one as well for comparison, so as to
detect when has gone off too much.
Regards Mike
-----Original Message-----
From: jennifer.hoover@pharma.Novartis.com
<jennifer.hoover@pharma.Novartis.com>
To: Histonet@Pathology.swmed.edu <Histonet@Pathology.swmed.edu>
Date: Saturday, 3 July 1999 8:00
Subject: Pancreatic Islet cells!


>     Hi Histonetters!  I am seeking input for Aldehyde Fucshin staining of
islet
>cells.  Currently I am doing histological work on 10% NBF fixed paraffin
>embedded mouse kidneys in which islet cells (also mouse) have been
transplanted
>under the renal capsule.  I have consulted Sheehan's book for  Gomori's
Aldehyde
>Fucshin staining of pancreatic islet cells but have had little success. The
>stain functions beautifully for the elastin tissue in the vasculature but
there
>is no staining of islet cells; even in normal pancreas tissue.  I know that
the
>stain needs to be prepared fresh and is good for only a few weeks. I have
also
>substituted acetaldehyde for paraldehyde in preparing the stain.  Still no
good
>staining of islets!  Any suggestions would be most greatly appreciated!
Thanks
>in advance!
>
>
>Jennifer Hoover
>Histologist
>Transplantation Biology
>Novartis Pharmaceuticals
>Summit, NJ
>
>
>
>