Re: Ki-67 (MIB-1)

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From:Dana Settembre <settembr@UMDNJ.EDU>
To:Richard Maliniak <>
Date:Tue, 13 Jul 1999 07:41:00 -0400 (EDT)
Content-Type:TEXT/PLAIN; charset=US-ASCII

On Mon, 12 Jul 1999, Richard Maliniak wrote:

> Does anyone have a protocol for Ki-67 (MIB-1) on formalin fixed paraffin
> embedded tissue?
> Thanks in Advance!
> Rick

Hi Rick,
I routinely use Dako's Ki-67 and have used Coulter's Ki-67 MIB-1. Both I
retrieve with BioGenex'citra buffer for a total of 30min in the microwave.(3min at 80% and 27min
at 20%)  I use Dako's at 1 :50 and Coulter's at 1:25 and use Dako's LSAB2
detection kit.  I have a Ki-67 positive tonsil for a control.
Works well.
Good luck

Dana Settembre
Immunohistochemistry Lab
University Hospital
Newark, NJ

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