Re: Ki-67 (MIB-1)
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| From: | Dana Settembre <settembr@UMDNJ.EDU> |
| To: | Richard Maliniak <maliniakrm@worldnet.att.net> |
| Reply-To: | |
| Date: | Tue, 13 Jul 1999 07:41:00 -0400 (EDT) |
| Content-Type: | TEXT/PLAIN; charset=US-ASCII |
On Mon, 12 Jul 1999, Richard Maliniak wrote:
> Does anyone have a protocol for Ki-67 (MIB-1) on formalin fixed paraffin
> embedded tissue?
>
> Thanks in Advance!
>
> Rick
>
Hi Rick,
I routinely use Dako's Ki-67 and have used Coulter's Ki-67 MIB-1. Both I
retrieve with BioGenex'citra buffer for a total of 30min in the microwave.(3min at 80% and 27min
at 20%) I use Dako's at 1 :50 and Coulter's at 1:25 and use Dako's LSAB2
detection kit. I have a Ki-67 positive tonsil for a control.
Works well.
Good luck
Dana Settembre
Immunohistochemistry Lab
University Hospital
Newark, NJ
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