Re: Freezing LARGE muscle specimens

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From:Leona Cohen-Gould <>
To:Margaret Gondo <>,
Date:Wed, 14 Jul 1999 12:23:50 -0400
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Dear Margaret,
20%sucrose in PBS works well in chick heart muscle.  The intact heart is
not as big as what you dal with, but getting the sucrose in there really
helps.  I also then infuse with a1:1mixture of  the sucrose and OCT before
embedding in either OCT or the surcors/OCT mixture (see Barthel & Raymond J
Histochem Cytochem 38(9)p1383).


Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Confocal Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice  (212)746-6146
fax (212)746-8175

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