Re: Freezing LARGE muscle specimens
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| From: | Leona Cohen-Gould <lcgould@mail.med.cornell.edu> |
| To: | Margaret Gondo <gondom@valentis.com>, Histonet@Pathology.swmed.edu |
| Reply-To: | |
| Date: | Wed, 14 Jul 1999 12:23:50 -0400 |
| Content-Type: | text/plain; charset="us-ascii" |
Dear Margaret,
Cryoprotect!!!
20%sucrose in PBS works well in chick heart muscle. The intact heart is
not as big as what you dal with, but getting the sucrose in there really
helps. I also then infuse with a1:1mixture of the sucrose and OCT before
embedding in either OCT or the surcors/OCT mixture (see Barthel & Raymond J
Histochem Cytochem 38(9)p1383).
Lee
Leona Cohen-Gould, M.S.
Sr. Staff Associate
Director, Electron Microscopy Core Facility
Manager, Confocal Microscopy Core Facility
Joan & Sanford I. Weill Medical College
of Cornell University
voice (212)746-6146
fax (212)746-8175
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