Hi Louise and other readers,

The E-Cadherin #08-1222 in our IHC catalog is designed for paraffin and a
whole lot easier to use. You might want to give it a try instead of
#13-1700. Most people that have tried the #08-1222 have switched.  Please
contact me if you have any questions.

Best Regards!

Nancy Shellhorn
At 09:23 AM 7/2/99 +1200, Maree Gould wrote:
>Hi Louise
>
>Not the easiest antibody I have worked with, but Zymed gave excellent
>results (cat 13-1700).
>I used gastric mucosa as a positive/negative control and the staining
>pattern is very distinctive.
>
>There is a Ref: Antigen retrieval solution recommended in Shi SR et al 1996
>Biotechnic and Histochem v71:4 190-196.
>
>Do not use picric acid to try to remove formalin pigment as I did, as the
>background staining became intense!  The tissue was fixed (a long time ago)
>in unbuffered formalin. I have removed formalin pigment with other
>antibodies with no problems.
>
>Any other questions, feel free to contact me.
>Maree Gould.
>
>_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/
>
>Maree Gould NZCS Dip Sci
>Histology Unit Pathology Dept
>Medical School
>University Of Otago
>P.O. Box 913
>Dunedin
>New Zealand
>maree.gould@stonebow.otago.ac.nz
>phone: 479 7152  Fax 479 7136
>
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>
>
>
>
Nancy Shellhorn 
Zymed Laboratories, Inc.
1-800-874-4494 ext. 252
904-249-6968 Fax:904-249-6441