I routinely do Desmin IHC on mice without any problems at all. As you
already noted, HIER diminishes labeling.  I use Dako's D33 AB 1:1000 for
30 minutes at room temp. If you need anymore info, just email back
directly.
Denise Long Woodward
dlongwoodward@bics.bwh.harvard.edu


On Tue, 29 Jun 1999 15:49:04 -0400 Greg Dobbin <dobbin@Upei.CA> writes:
>Hello All,
>
>I'll be REALLY glad when the IHC in mice and rats info. is compiled!
>
>I am trying to get mouse tissue to stain for desmin; with minimal
>success so far. There is just a hint of a positive reaction, and
>surprisingly it is slightly better without ag retreival (tissues are
>FFPE- 5um). However the rx is just too weak overall. Is there
>anything peculiar about IHC staining for desmin (in mice) that
>I should know about? (I used citrate buffer in steam for HIER
>and a polyclonal, rabbit anti-desmin).
>
>                                                             Cheers!
>Greg
>>
>>
>>
>>
>>
>>
>Greg Dobbin
>Pathology Lab
>Atlantic Veterinary College, U.P.E.I.
>550 Unviversity Ave.
>Charlottetown, P.E.I.
>C1A 4P3
>Phone: (902)566-0744
>Fax: (902)566-0851
>

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