Re: Collagen Type VII and BP Ab's
<< Previous Message | Next Message >>
From: | Mbbiocare@aol.com |
To: | burch007@mc.duke.edu, HistoNet@Pathology.swmed.edu, lucila_gonzalez@srhc.iwhs.org |
Reply-To: | |
Date: | Wed, 7 Jul 1999 14:07:57 EDT |
Content-Type: | text/plain; charset="us-ascii" |
Back in 1993 I published an abstract in Lab Investigation Vol70#1 Page 69A
1994 "Immunohistochemistry of Type V11 Collagen in The Prostate. At that
time we published data on methyl-Carnoys fixative Clone LH7.2 which I had
gotten from Biodesign Int., Kennebunkport, CA I did a dilution of 1:300
for 2hrs at RT.
Later however I did some studies using FFPE tissue using an overnight
incubation and it worked fine. I also did some post fixing of FFPE slides in
Carnoy's overnight. I would have to try and find my records to give you any
details. Since you have the antibody from Chemicon, my suggestions are to try
the overnight incubation with primary and use Biocare Medical's detection
system with their DAB Sparkle, as I had also enhanced my DAB. Their
detection system is very sensitive and I have seen slides that are really
beautiful using their system. Give Vygis Narbutas a call at 800-799-9499
There is a money back guarantee on all their products.
Marianne
<< Previous Message | Next Message >>