Re: Cleaning the microtome
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From: | "R.Wadley" <s9803537@pop3.unsw.edu.au> |
To: | HistoNet@Pathology.swmed.edu |
Reply-To: | |
Date: | Mon, 05 Jul 1999 08:28:46 +1000 |
Content-Type: | text/plain; charset="us-ascii" |
Dear Andrea,
I was taught (& always practice) to clean my microtome every day at the
end of the cutting session. A moderately stiff paint brush a couple of cm
wide does the trick. If I have a long cutting session I will clean away
excess wax at intervals as it builds up.
If there is a build up of wax then the use of a plastic scraper (never
metal) may be required. As a last resort a little absolute ethanol on a
tissue does wonders to shift any remaining wax.
A clean microtome means better sections are cut faster.
I had a junior technician who insisted on wiping down the microtome with
xylene. This is a practice I strongly advise against.
1/ It exposes the operator to way too many potentially toxic fumes.
2/ She never wore gloves & xylene is a contact allergen.
3/ You should never get that much wax stuck to the instrument if its
cleaned regularly every day.
4/ You will remove all the grease & oil from the areas it is required.
Regards
Rob.
At 01:29 PM 7/2/99 GMT, you wrote:
>I was wondering how people keep their microtomes clean? What do you use to
>clean it and how often? Every day? Biweekly etc?
R. Wadley, B.App.Sc, M.L.S
Laboratory Manager
Cellular Analysis Facility
School of Microbiology & Immunology
UNSW, New South Wales, Australia, 2052
Ph (BH) +61 (2) 9385 3517
Ph (AH) +61 (2) 9555 1239
Fax +61 (2) 9385 1591
E-mail r.wadley@unsw.edu.au
www http://www.micro.unsw.edu.au/caf.html
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