Re: CD8

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From:Dana Settembre <settembr@UMDNJ.EDU>
To:Ginny Achstetter <>
Date:Tue, 6 Jul 1999 12:42:54 -0400 (EDT)
Content-Type:TEXT/PLAIN; charset=US-ASCII

On Fri, 2 Jul 1999, Ginny Achstetter wrote:

> What kind of pretreatment is done for CD8.  Microwaving? Digestion?
> Thanks.

Hello Ginny,
I use citra buffer in the microwave, 3min at 80% and 7min at 20% for a
total of 10minutes.  I use BioGenex' Citra 10X cat.#HK086-9K with
Dako's CD8 cat# M7103 on human tissue.  Hope this helps.

Dana Settembre
Immunohistochemistry Lab
University Hospital
Newark, NJ

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