RE: destaining of special stain and restaining with ER/PR

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From:"Carson, Karla" <KCarson@chw.edu>
To:"'Donna Sitrin'" <dsitrin@unipathllc.com>, <histonet@Pathology.swmed.edu>
Reply-To:
Date:Thu, 15 Jul 1999 06:48:24 -0700
Content-Type:text/plain; charset=us-ascii

I am trying the other way and am having trouble completely removing the
Diff-Quik before the WG.  I too could use help.  
Karla Carson HT/HTL(ASCP)
Regional Pathology Supervisor	
Mercy Healthcare Sacramento
916-453-4494
kcarson@chw.edu <mailto:kcarson@chw.edu> 

	-----Original Message-----
	From:	Donna Sitrin [SMTP:dsitrin@unipathllc.com]
	Sent:	Wednesday, July 14, 1999 3:48 PM
	To:	histonet@pathology.swmed.edu
	Subject:	destaining of special stain and restaining with
ER/PR

	I'm working on an important project that is going to require the
destaining
	of an FNA smear(Wright-Giemsa).
	There may not be any acid alcohol components available, and this
will need
	to be restained with a Diff Quik and possibly with an ER/PR.  Does
anyone
	have any words of wisdom as far as the best method of destaining
that will
	not thoroughly destroy receptor
	sites?

	Thank you so much. This is a humanitarian effort.

	Donna Sitrin
	Unipath LLC, Denver

	




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