RE: Re: A quickmorphology stain for paraffin sections?

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From:"margaret blount" <>
To:"Tim Morken" <>, "" <>
Date:Thu, 1 Jul 1999 09:34:46 +0100

Another trick is to look at the block face under a stereo microscope.
I have done this for very small specimens.
Margaret Blount
Unilever research

-----Original Message-----
From:	Tim Morken []
Sent:	Wednesday, June 30, 1999 8:46 PM
Subject:	Re: A quickmorphology stain for paraffin sections?


If the structure is fairly large you can use a pseudo-interference contrast 
illumination method to see structure in the section. Just move the objective 
of the microscope slightly to one side of its normal position and you can 
see 3D structure without doing any deparaffinizing or staining. You will be 
surprised how much detail you can make out. This is a great method for 
finding gloms on kidney frozens.

Tim Morken, B.A., EMT(MSA), HTL(ASCP)
Infectious Disease Pathology
Centers for Disease Control
1600 Clifton Rd.
Atlanta, GA 30333


Phone: (404) 639-3964
FAX:  (404)639-3043

----Original Message Follows----
From: "Barry Rittman" <>
To: histonet <>
Subject: Re: A quickmorphology stain for paraffin sections?
Date: Wed, 30 Jun 1999 12:15:28 -0500

                 we used to use a cotton ball moistened with dilute 
blue to wipe over the surface of the block. This gave us a good idea of the
tissue at that level and helped greatly in the orientation.
If you prefer  you can always place a cut section on a slide and add several
drops of dilute aqueous methylene blue (say 0.05-0.1%), this also works 
No need to mount the section.
Barry wrote:

 > This is probably a silly question but ... I was wondering is there any 
 > to quickly stain paraffin sections so that I can evaluate whether or not 
 > need to cut further into the block.  We are looking for something very
 > specific and every section counts but to section the whole block would 
 > hours of time not to mention many many expensive slides?  Can you quick
 > stain sections without deparaffinizing?
 > Andrea

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