RE: Pancreatic Islet cells!

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From:"Kellar, Eric" <kellarec@MSX.UPMC.EDU>
To:"' '" <>, "' '" <>
Date:Fri, 2 Jul 1999 19:49:41 -0400
Content-Type:text/plain; charset="windows-1252"

The Aldehyde fuchsin solution must be allowed to ripen for approximately one
week (3 to 7 days) before use for improved staining results.

Eric C. Kellar
University of Pittsburgh Medical Center 

-----Original Message-----
Sent: 7/2/99 10:18 AM
Subject: Pancreatic Islet cells!

     Hi Histonetters!  I am seeking input for Aldehyde Fucshin staining
of islet
cells.  Currently I am doing histological work on 10% NBF fixed paraffin
embedded mouse kidneys in which islet cells (also mouse) have been
under the renal capsule.  I have consulted Sheehan's book for  Gomori's
Fucshin staining of pancreatic islet cells but have had little success.
stain functions beautifully for the elastin tissue in the vasculature
but there
is no staining of islet cells; even in normal pancreas tissue.  I know
that the
stain needs to be prepared fresh and is good for only a few weeks. I
have also
substituted acetaldehyde for paraldehyde in preparing the stain.  Still
no good
staining of islets!  Any suggestions would be most greatly appreciated!
in advance!

Jennifer Hoover
Transplantation Biology
Novartis Pharmaceuticals
Summit, NJ

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