From: | "Kellar, Eric" <kellarec@MSX.UPMC.EDU> |
To: | "'jennifer.hoover@pharma.Novartis.com '" <jennifer.hoover@pharma.Novartis.com>, "'Histonet@Pathology.swmed.edu '" <Histonet@Pathology.swmed.edu> |
Reply-To: | |
Date: | Fri, 2 Jul 1999 19:49:41 -0400 |
Content-Type: | text/plain; charset="windows-1252" |
The Aldehyde fuchsin solution must be allowed to ripen for approximately one week (3 to 7 days) before use for improved staining results. Eric C. Kellar Histology/Immunohistochemistry University of Pittsburgh Medical Center -----Original Message----- From: jennifer.hoover@pharma.Novartis.com To: Histonet@Pathology.swmed.edu Sent: 7/2/99 10:18 AM Subject: Pancreatic Islet cells! Hi Histonetters! I am seeking input for Aldehyde Fucshin staining of islet cells. Currently I am doing histological work on 10% NBF fixed paraffin embedded mouse kidneys in which islet cells (also mouse) have been transplanted under the renal capsule. I have consulted Sheehan's book for Gomori's Aldehyde Fucshin staining of pancreatic islet cells but have had little success. The stain functions beautifully for the elastin tissue in the vasculature but there is no staining of islet cells; even in normal pancreas tissue. I know that the stain needs to be prepared fresh and is good for only a few weeks. I have also substituted acetaldehyde for paraldehyde in preparing the stain. Still no good staining of islets! Any suggestions would be most greatly appreciated! Thanks in advance! Jennifer Hoover Histologist Transplantation Biology Novartis Pharmaceuticals Summit, NJ