RE: Fixed frozen section adhesion
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From: | Alex Brown <AlexB@nayrshire.scot.nhs.uk> |
To: | "'Histonet'" <Histonet@Pathology.swmed.edu> |
Reply-To: | |
Date: | Wed, 30 Jun 1999 19:27:00 +0100 |
Content-Type: | text/plain; charset="ISO-8859-1" |
Hi John,
Never touch rat brains if I can help it ( although one never
really knows what's in a curry :¬) ) but we occasionally do Oil Red
O's on fixed frozen human tissue.
We pick up the sections on 'charged' slides and lay them on a
slide tray and let them dry for a few minutes ( or longer, - no fixed
time really ) We then put them in a microwave for 10 secs on 'high' (
I know this vagueness will really annoy a certain Mr Slap - Sorry! )
before 'staining' . I have no idea why this makes a difference, but we
found it helped to keep the sections on the slide. Any enlightenment
welcome.
Hope this might help,
Alex Brown
Crosshouse Hospital
Kilmarnock, Scotland.
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