remove me from this mailing list
thanks


> -----Original Message-----
> From: HistoNet Server [mailto:HistoNet@Pathology.swmed.edu]
> Sent: Thursday, July 08, 1999 11:54 PM
> To: HistoNet Server
> Subject: Daily Digest
>
>
>
> ----------------------------------------------------------------------
>
> Date: 8 Jul 1999 09:17:59 -0500
> From: Geoff McAuliffe <mcauliff@UMDNJ.EDU>
> Subject: Re: The future of the Histology lab?
>
> Tim Morken wrote:
>
> > Histonetters,
> >
> > A recent article in the June 1999 CAP Today (U.S. only),
> "Virtual Tissue,"
> > and the web site www.adpath.com give one possible version of
> the histology
> > lab 5 to 20 years from now (depending on whether you are in a
> research or
> > clinical lab). The article has sample images, the web site only has an
> > explaination of the technology about to hit the histology lab.
> >
> > In short, a piece of pre-stained, resin-embedded tissue is cut into
> > thousands of sections by an automated microtome. Some sections
> are kept for
> > specialized studies but the vast majority are discarded.
> Instead of using
> > the sections, a digital image is made of the block face after
> each section
> > and a 3D digital image of the entire tissue is produced.  Using
> your desktop
> > computer you can then "section" the 'virtual tissue' in the most useful
> > plane for diagnosis.
> >
> > Stains are applied before embedding using fluorescent dyes for
> the common
> > tissue elements. These "stains" can then be interpreted by computer
> > enhancement to produce the "stains" a pathologist is used to seeing.
> >
> > Seems that with DVD technology a microscopical 3D digital image of the
> > entire tissue sample from a case can be stored.
> >
> > If you wonder how it looks, an "H&E" by this method is as good or better
> > than a real H&E. It certainly has better detail.
> >
> > I think the histology lab is about to be 'all shook up.' Hope
> you all are
> > ready!
> >
> > Tim Morken, B.A., EMT(MSA), HTL(ASCP)
> > Infectious Disease Pathology
> > Centers for Disease Control
> > MS-G32
> > 1600 Clifton Rd.
> > Atlanta, GA 30333
> > USA
> >
> > email: tim9@cdc.gov
> >        timcdc@hotmail.com
> >
> > Phone: (404) 639-3964
> > FAX:  (404)639-3043
>
> Dear Histonetters:
>
>     So where will the money to buy all of this wonderful stuff
> come from (once
> the bugs are out of this wonderful stuff and it is on the market)? Are
> granting
> agencies going to give out buckest of bucks for 3-D
> reconstruction which is
> already possible, albeit more slowly? To what end? I think laser scanning
> confocal will already do many of the tasks mentioned above. I
> can't speak for
> clinical labs but from the postings I read on this list, it seems money is
> pretty tight there, too. How would this improve diagnosis?
>     Don't get me wrong, it would be wonderful to be able to do
> these things
> quickly and easily, but I doubt that it will be widespread. Maybe
> at NIH or a
> big drug house. These methods were used to create the Virtual Man
> and Woman at
> the NIH but I teach Gross Anatomy and I don't know a single US
> medical school
> that is or plans to do this on site.
>     I remember being told in the '70's that paraffin was going to
> be obsolete
> in
> a few years, we would all be doing resin sections.
>
> Geoff
> - --
> **********************************************
> Geoff McAuliffe, Ph.D.
> Neuroscience and Cell Biology
> Robert Wood Johnson Medical School
> 675 Hoes Lane, Piscataway, NJ 08854
> voice: (732)-235-4583; fax: -4029
> mcauliff@umdnj.edu
> **********************************************
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 8 Jul 1999 09:38:43 -0500
> From: Angel92764@aol.com
> Subject: Fwd: PARA / GARD
>
>
>
>
>
>
> ******************* NOTE *******************
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> contained in the following MIME Information.
> ********************************************
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> Return-path: Angel92764@aol.com
> From: Angel92764@aol.com
> Full-name: Angel92764
> Message-ID: <14de1fc4.24b4e20e@aol.com>
> Date: Wed, 7 Jul 1999 13:02:06 EDT
> Subject: PARA / GARD
> To: HistoNet@Pathology.swmed.edu
> MIME-Version: 1.0
> Content-Type: text/plain; charset="us-ascii"
> Content-Transfer-Encoding: 7bit
> X-Mailer: AOL 4.0 for Windows 95 sub 216
>
> Anyone who was interested in PARA/GARD, it is manufactured by Triangle
> Biomedical Sciences (TBS) in Durham, NC.  It comes in a 4oz
> bottle and is a
> paraffin repellent for use on microtomes and countertops.  The
> catalog # is
> H-PG.  We purchase ours through Info-lab.  When we clean our
> microtomes, we
> just brush off the debris, and spray the microtome with para/gard
> and then
> wipe with a paper towel.  If you try this, I feel sure you will like it.
>
>
> 	Jeanie Wade, H.T. (ASCP)
>
> - --part1_14de1fc4.24b548fe_boundary--
>
>
> ----------------------------------------------------------------------
>
> Date: 8 Jul 1999 09:39:32 -0500
> From: Tracy Montag <tracy@cvs.rochester.edu>
> Subject: resubscribe
>
> Please resubscribe
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 8 Jul 1999 09:40:27 -0500
> From: Victoria Baker <vbaker60@yahoo.com>
> Subject: Has anyone received messages?
>
> Hi!
>
> It's been a bit since I have received any messages from the list
> server, has this happened to anyone else?
>
> Vikki Baker
>
>
> _________________________________________________________
> Do You Yahoo!?
> Get your free @yahoo.com address at http://mail.yahoo.com
>
>
>
> ----------------------------------------------------------------------
>
> Date: 8 Jul 1999 09:41:25 -0500
> From: "Jaci Lett" <jmlett@cid.wustl.edu>
> Subject: TEM:  Heat pen for flattening sections
>
> Does anyone have any opinions on using a heat pen to flatten ultrathin EM
> sections?  I would very much prefer to get away from using chloroform.
>
> Electron Microscopy Sciences has two models:  Wax Pen 1 (which uses 1 AA
> battery) and Wax Pen 2 (which uses 2 AA batteries).  Their tech
> representative recommended the Wax Pen 2 for flattening sections.  Is this
> consistent with the preference of the majority?
>
> I need to decide to place an order soon (the end of the fiscal year is
> approaching).
>
> Thank you,
>
> Jaclynn Lett, Research Assistant     jmlett@cid.wustl.edu
>
> Fay and Carl Simon Center for the Biology of Hearing and Deafness
> Central Institute for the Deaf
> 818 S. Euclid Ave.
> St. Louis, MO 63110
>
> voice  314-977-0257     fax  314-977-0030
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 8 Jul 1999 09:41:50 -0500
> From: Andrea Kaj <andreakaj@hotmail.com>
> Subject: PFA frozens
>
> Hi Gayle
> I work with PFA fixed frozen mouse and rat tissue. What I usually do is
> embedding the (wiped off) fixated tissue in OTC, put it in marked plastic
> containers and simply put in in the -80 freezer. The tissue can withstand
> several freeze-'thaw' cycles, by thawing I mean bringing the
> tissue to the
> cryostat (-22) and back to the freezer (tranported in liquid nitrogen).
> Kind regards Andrea
>
>
> ______________________________________________________
> Get Your Private, Free Email at http://www.hotmail.com
>
>
> ----------------------------------------------------------------------
>
> Date: 8 Jul 1999 09:42:17 -0500
> From: "Susan Cohane" <susan_cohane@millet.com>
> Subject: F.Y.I. unsubscribe
>
>
>     7/7/99
>
>     8:19 AM
>
> F.Y.I. unsubscribe
> unsubscribe.
>
>
> Susan M. Cohane
> Senior Account Manager
> Lehman Millet Incorporated 617.722.0019
> 1.800.634.5315
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 8 Jul 1999 09:42:49 -0500
> From: Andrea Kaj <andreakaj@hotmail.com>
> Subject: Mouse tissue
>
> Hi Karen
> I have worked a lot with frozen mouse tissue, especially intestine from
> foetus. I always use silane coated slides (homemade), and I leave
> the tissue
> to air dry over night (if it is not fixated, you could fixate it
> in acetone
> for 5 min. after cutting and then let them air dry). I have the
> best result
> when fixating the tissue in 4% PFA acouple of hours in the
> fridge. Hope this
> helps.
> Kind regards Andrea
>
>
> ______________________________________________________
> Get Your Private, Free Email at http://www.hotmail.com
>
>
> ----------------------------------------------------------------------
>
> Date: 8 Jul 1999 09:43:21 -0500
> From: Alex Szabo <aszabo@gribbles.com.au>
> Subject: ISH Gene Bank Database
>
> The gene bank database is located at
>
> www2.ncbi.nlm.nih.gov/cgi-bin/genbank
>
> There you can design your target probes and check whether they have any
> significant homology with other sequences that may be present.
>
> When you are happy with your probe, order them from LIFE
> TECHNOLOGIES, Inc,
>  at
>
> www.lifetech.com
>
> 9800 Medical Center Drive
> Post Office Box 6482
> Rockville, MD 20849-6482, USA
> 1-800-338-5772
>
> Hope this helps
>
> Alex Szabo
>
>
>
> ----------------------------------------------------------------------
>
> Date: 8 Jul 1999 09:44:30 -0500
> From: "Patrick M. Haley " <pmhales@cybergap.net>
> Subject: Hello?
>
> Is the server down? It has been quiet.
>
> Pat Haley
>
>
>
> ----------------------------------------------------------------------
>
> Date: 8 Jul 1999 09:44:56 -0500
> From: maliniakrm@worldnet.att.net
> Subject: Stat Immunoperoxidases
>
> Did anyone attend...."The Mississippi Society for Histotechnology will
> hold the annual spring meeting on May 22, 1999, at Isle of Capri Casino
> in Biloxi, MS?
> I am interested in the lecture "Stat immunostains in under one hour"
> presented by Zahra Nassar of Innovex. Did the procedure produce good
> result?
>
> Rick
>
>
> ----------------------------------------------------------------------
>
> Date: 8 Jul 1999 09:45:41 -0500
> From: "Todd Sherman" <tsherman@flash.net>
> Subject: RE: Inexpensive Antibodies
>
> Hello Rick,
>
> We've not purchased primary antibodies from this company but have
> purchased
> other products...so, yes, they are still around.  Check
> http://www.cellmarque.com/ .  I believe they are located in/around Austin,
> TX.
>
> Todd
>
>
> Todd Sherman
> UT Southwestern Medical Center
> Pediatrics/Neonatal Medicine
> todd.sherman@email.swmed.edu
>
> - -----Original Message-----
> From: Richard Maliniak [mailto:maliniakrm@worldnet.att.net]
> Sent: Tuesday, July 06, 1999 7:04 PM
> To: HistoNet
> Subject: Inexpensive Antibodies
>
>
> I am currently looking for the lowest possible costs for concentrated
> antibodies to use on a DAKO autostainer.  I remember a few years ago a
> company called...Cell Marque {spelling?} had low prices.  Are they still
> around?
>
> Rick
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 8 Jul 1999 09:46:16 -0500
> From: Heike Grabsch <h.grabsch@uni-koeln.de>
> Subject: htert-antibody
>
> Calbiochem now sells an antibody against htert, the catalytic subunit of
> telomerase. Is there any body out there in histoland who already tried it
> on paraffin sections?
>
> Thanks for your help,
>
> Dr. Heike Grabsch
> Dep. of Pathology
> University of Duesseldorf
> Moorenstrasse 5
> 40225 Duesseldorf
> Germany
> another email is grabsch@med.uni-duesseldorf.de
>
>
> ----------------------------------------------------------------------
>
> Date: 8 Jul 1999 09:46:49 -0500
> From: "Robin E. Taylor" <ret@gene.com>
> Subject: Flexible Colloidin
>
>
> I have been searching for a reference and article on using flexible
> colloidin for making cell pellets - does anyone know of this method (and
> have the article)?
>
> I have tried literature searches and have come up empty.
>
> Please email me directly.
>
> Thanks.
>
> Robin
>
>
>
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> contained in the following MIME Information.
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> begin:vcard
> n:Taylor;Robin E.
> tel;fax:650.225.6835
> tel;work:650.225.2823
> x-mozilla-html:FALSE
> org:Genentech, Inc.;Pathology
> adr:;;MS6, 1 DNA Way;South San Francisco;CA;94080;USA
> version:2.1
> email;internet:ret@gene.com
> title:Scientific Manager, Anatomic Pathology
> x-mozilla-cpt:;1
> fn:Robin E. Taylor
> end:vcard
>
> - --------------E260E29822698600B31098DD--
>
>
>
> ----------------------------------------------------------------------
>
> Date: 8 Jul 1999 09:47:17 -0500
> From: rschoonh@sph.unc.edu
> Subject: PGST antibody woes
>
> HistoNetters'
>
> I am having a difficult time locating anti PGST (Placental Glutathione
> s-Transferase).
>
> I've been told that Novaa Castra has it and I thought that Zymed
> distributes their antibodies here in the U.S. but when I called them
> they could't locate the product :-(    Of course I needed this
> yesterday.    T.I.A.
>
> best regards,
> Bob
> Robert Schoonhoven
> Laboratory of Molecular Carcinogenesis and Mutagenesis
> Dept. of Environmental Sciences and Engineering
> University of North Carolina
> CB#7400
> Chapel Hill, NC 27599
> Phone
> office 919-966-6343
>    Lab 919-966-6140
>    Fax 919-966-6123
>
> Don't go around saying the world owes you a living; the world owes you
> nothing; it was here first.
> Mark Twain [Samuel Langhornne Clemens] (1835-1910)
>
>
>
> ----------------------------------------------------------------------
>
> Date: 8 Jul 1999 09:48:09 -0500
> From: Ian Montgomery <ian.montgomery@bio.gla.ac.uk>
> Subject: FW: Freezing muscle sections/snap freeing technique
>
> >Date: Fri, 2 Jul 1999 17:55:47 +1000
> >From: jim <jim@proscitech.com.au>
> >Subject: FW: Freezing muscle sections/snap freeing technique
> >To: "'Tim Fairchild'" <timf@cyllene.uwa.edu.au>,
> >        HistoNet Server
> >	 <HistoNet@pathology.swmed.edu>
> >Mime-Version: 1.0
> >
> Jim,
> 	You got me when I was a wee bit nippy. Family trait, crabbit, bad
> tempered and quick to reach for the Lochaber axe. I'll buy you a
> pint first
> time we meet.
>
> >Tim - Isopentane is not the best medium to use!
> 	Have to disagree, for good muscle histochemistry, the original
> question, isopentane cooled with liquid nitrogen is essential. Ok, I have
> used talc coated specimens in the past, but that was only in an emergency.
>
> >Isopentane is very sticky and almost unusable when its near liq nitrogen
> >temperature.
> 	Isopentane is completely unusable at liquid nitrogen temperature,
> you have a solid block. Not much use for plunge freezing.
>
> Warmed up (with a metal rod) the cooling rate of the specimen is
> >reduced, because (heat transfer rates of various media aside and liquid
> >nitrogen is lousy, because it forms an "insulating" gas layer),
> the colder
> >the
> >medium the better.
> 	Ok.
> >
> >The second rule is that smaller specimens freeze better and the out-most
> >part of the specimen will be best preserved. Flat specimens are suitable.
> 	Small is lovely, but remember we are dealing with LM Histochemistry
> and not EM. Orientation of a flat piece of TS muscle is a bummer,
> 'tall and
> slim or short and squat is in'
> >
> >Third, specimens with lower water contents will show less
> damage. So, skin
> >(or
> >cork!) will be less affected by ice crystal formation than liver.
> > Ice crystal damage can be reduced by fixing and then infusing
> with say 20%
> >glycerin, but this may defeat your reasons for cryo.
> 	Ok, but for muscle enzyme histochemistry a wee whiff of fixatives
> is the kiss of death.
>
> >A very good freezing medium is liquid propane, cooled by liq
> nitrogen like
> >your
> >isopentane.
> >Use a fumehood throughout. Prepare your cold-cup with liquid
> nitrogen. Have a
> >needle (cut off square 19 gauge or similar) inserted into a bit of tygon
> >tubing
> >connected to the outlet valve of a small propane gas cylinder (without
> >regulators; purity of gas is not very important). Sit the cylinder
> >up-side-down
> >so liquid is expelled (preferred but works either way).
> 	Propane is a good cryogen, but again this is muscle histochemistry
> and not EM.
> 	WITHOUT REGULATORS, in the name of the wee man, Safety Officers
> would go totally ballistic without a back flow/flame regulator.  -180
> atmospheric oxygen dissolves into the cold propane and what a combination
> that is and no regulator to stop the back flow into the propane tank. I
> agree the purity of the propane is not important, in fact impurities are
> good. I use a stirred mixture of isopentane and propane in order
> to depress
> the temperature and keep it away from -180 and the naughty effects of
> oxygen.
> >
> >Open the valve very slowly so gas can only just be feeled on
> skin. Rub needle
> >gently in the cold cup. The emitted gas will turn into liquid.
> >Snap freeze specimens by very rapid immersion movement. The best freezing
> >happens in a fraction of a second and largely depends on the temperature
> >differential between specimen and the medium; since the interface warms
> >rapidly, the rapid movement is required.
> >Also assure that the transfer into liquid nitrogen for specimen
> storage is
> >very rapid.
> 	Ok. Although I agree propane is very good for cryo EM, the best
> cryo-preservation I have ever obtained was with a specimen slammed on a
> cooled ultra pure copper block and freeze-substituted. I even published a
> micrograph in the Proceedings of the RMS so happy was I. Muscle
> histochemistry, isopentane cooled with liquid nitrogen is the business.
>
> >Hope this helps.
> >Cheers
> >Jim Darley
> >
> >ProSciTech                 Microscopy PLUS
> >PO Box 111, Thuringowa  QLD  4817  Australia
> >Ph +61 7 4774 0370  Fax:+61 7 4789 2313  service@proscitech.com.au
> >Great microscopy catalogue, 500 Links, MSDS, User Notes
> >                      www.proscitech.com.au
> >> -----Original Message-----
> >> From:	Tim Fairchild [SMTP:timf@cyllene.uwa.edu.au]
> >> Sent:	Thursday, July 01, 1999 3:14 AM
> >> To:	HistoNet Server
> >> Subject:	Freezing muscle sections
> >>
> >> We have recently undertaken a project which required a portion
> of muscle
> >> to be analysed for fibre type and oxidative capacity.  The technique we
> >> adopted to freeze the muscle (human muscle), was to mount the muscle on
> >> cork using 'gum tragacanth', and then freezing this in
> isopentane cooled
> >> in liquid nitrogen.  The trouble we're having is that every 5th sample
> >> (roughly speaking) has ice crystal artifact through it.  I am
> >> attributing this to the isopentan not being cold enough.  I guess my
> >> questions therefore are:
> >>
> >> 1. Is there a way to protect the muscle from the freezing process, i.e.
> >> putting O.C.T. over the muscle?
> >> 2. If the muscle has to be frozen in isopentane, what 'set up' has
> >> worked for other people (i.e. we put the isopentane in a long metal
> >> cylindrical container, inserted in a larger container holding liquid
> >> nitrogen) and what techniques have you found useful (e.g. hold in
> >> isopentane for 20 seconds)?
> >>
> >> Any help (or small tips) would be very much appreciated!
> >>
> >> Thanks in advance,
> >>
> >> Tim Fairchild.
> >> -----------------------------------------------------------------
> >> Timothy J. Fairchild B.Sc. (Hons)
> >> PhD Candidate
> >> Co-ordinator for Centre of Athletic Testing
> >> Department of Human Movement and Exercise Science
> >> Nedlands, Western Australia 6907
> >> Telephone: (+61 8) 9380 2793
> >> Facsimile:  (+61 8) 9380 1039
> >> Email: timf@cyllene.uwa.edu.au
> >> http://www.general.uwa.edu.au/~hmweb/index.htm
> >>
> >>
> >>
> >
>
> Dr. Ian Montgomery,
> West Medical Building,
> University of Glasgow,
> Glasgow,
> G12 8QQ,
> Scotland.
> Tel: 0141 339 8855 Extn. 6602.
> Fax: 0141 330 4100.
> e-mail: ian.montgomery@bio.gla.ac.uk
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 8 Jul 1999 09:48:37 -0500
> From: Angel92764@aol.com
> Subject: PARA / GARD
>
> Anyone who was interested in PARA/GARD, it is manufactured by Triangle
> Biomedical Sciences (TBS) in Durham, NC.  It comes in a 4oz
> bottle and is a
> paraffin repellent for use on microtomes and countertops.  The
> catalog # is
> H-PG.  We purchase ours through Info-lab.  When we clean our
> microtomes, we
> just brush off the debris, and spray the microtome with para/gard
> and then
> wipe with a paper towel.  If you try this, I feel sure you will like it.
>
>
> 	Jeanie Wade, H.T. (ASCP)
>
>
> ----------------------------------------------------------------------
>
> Date: 8 Jul 1999 09:49:04 -0500
> From: <Kate_E_Rhodes@sbphrd.com>
> Subject: autoradiography (taggged w/ 14C
>
> Looking for information on development time for specimens tagged with 14C
> dipped with NTB2.  Although we are doing several time points, I have no
> idea what a starting development time would be-one week, two weeks, etc???
>
> Any help would be greatly appreciated.
>
> Thanks,
> Kate Rhodes
> SmithKline Beecham Pharmaceutical Co.
> 610-270-7340
> FAX 610-270-7202
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 8 Jul 1999 09:49:32 -0500
> From: Heike Grabsch <h.grabsch@uni-koeln.de>
> Subject: anti-MSH6
>
> Serotec now sells two different monoclonal antibodies against MSH6
> protein, however both of them were not tested in paraffin sections.
> Anybody out thre in histoland, who already tried whether it can be used
> in routine diagnostic?
>
> Thanks for your help
>
> Dr. Heike Grabsch
> Dep. of Pathology
> University of Duesseldorf
> Moorenstrasse 5
> 40225 Duesseldorf
> Germany
> another email grabsch@med.uni-duesseldorf.de
>
>
> ----------------------------------------------------------------------
>
> Date: 8 Jul 1999 09:50:11 -0500
> From: lynn_brennan@smtplink.Coh.ORG
> Subject: fix with IHC on brain
>
> What is the best fixative (you can make in the lab) using IHC and frozen
> sections on embryonic spinal cords?
> Any suggestions for the best fixative with any brain tissues?
> Many thanks,
> Lynn Brennan
> Division of Neurosciences
> City of Hope  Medical center
> 1500 Duarte Road
> Duarte,California  91010
>
>
>
> ----------------------------------------------------------------------
>
> Date: 8 Jul 1999 09:50:45 -0500
> From: "Weems, Joyce" <JWEEMS@sjha.org>
> Subject: RE: The future of the Histology lab?
>
> Elvis HAS left the building! I don't want to be "All shook up"! j:>)
> Joyce Weems
> Pathology Manager
> Saint Joseph's Hospital of Atlanta
>
>
> 	-----Original Message-----
> 	From:	Tim Morken [SMTP:timcdc@hotmail.com]
> 	Sent:	Tuesday, July 06, 1999 4:22 PM
> 	To:	histonet@Pathology.swmed.edu
> 	Subject:	The future of the Histology lab?
>
> 	Histonetters,
>
> 	A recent article in the June 1999 CAP Today (U.S. only), "Virtual
> Tissue,"
> 	and the web site www.adpath.com give one possible version of the
> histology
> 	lab 5 to 20 years from now (depending on whether you are in a
> research or
> 	clinical lab). The article has sample images, the web site only has
> an
> 	explaination of the technology about to hit the histology lab.
>
> 	In short, a piece of pre-stained, resin-embedded tissue is cut into
> 	thousands of sections by an automated microtome. Some sections are
> kept for
> 	specialized studies but the vast majority are discarded. Instead of
> using
> 	the sections, a digital image is made of the block face after each
> section
> 	and a 3D digital image of the entire tissue is produced.  Using your
> desktop
> 	computer you can then "section" the 'virtual tissue' in the most
> useful
> 	plane for diagnosis.
>
> 	Stains are applied before embedding using fluorescent dyes for the
> common
> 	tissue elements. These "stains" can then be interpreted by computer
> 	enhancement to produce the "stains" a pathologist is used to seeing.
>
> 	Seems that with DVD technology a microscopical 3D digital image of
> the
> 	entire tissue sample from a case can be stored.
>
> 	If you wonder how it looks, an "H&E" by this method is as good or
> better
> 	than a real H&E. It certainly has better detail.
>
> 	I think the histology lab is about to be 'all shook up.' Hope you
> all are
> 	ready!
>
>
>
> 	Tim Morken, B.A., EMT(MSA), HTL(ASCP)
> 	Infectious Disease Pathology
> 	Centers for Disease Control
> 	MS-G32
> 	1600 Clifton Rd.
> 	Atlanta, GA 30333
> 	USA
>
> 	email: tim9@cdc.gov
> 	       timcdc@hotmail.com
>
> 	Phone: (404) 639-3964
> 	FAX:  (404)639-3043
>
>
>
>
> 	_______________________________________________________________
> 	Get Free Email and Do More On The Web. Visit http://www.msn.com
>
>
> ----------------------------------------------------------------------
>
> Date: 8 Jul 1999 09:51:19 -0500
> From: Angel92764@aol.com
> Subject: Re: Cleaning the microtome/Para-gard
>
> We get our para-gard from info-lab.  I will have to get more
> information for
> you tomorrow at work.  If you have an info-lab distributor, they
> will be able
> to help you get some (maybe even a free sample).  This stuff is
> great.  It is
> MUCH better than xylene for cleaning your  microtome.  It is made
> specifically for cleaning microtomes and counter tops.  I highly
> recommend
> it.
>
>
>
> 	Jeanie Wade, H.T. (ASCP)
>
>
> ----------------------------------------------------------------------
>
> Date: 8 Jul 1999 09:51:58 -0500
> From: HACKERLAB@aol.com
> Subject: Dry areas on coverslips
>
> Attn: Pat Karlisch
> Geisinger Med Cr
> Danville, PA
>
> RE: Your e-mail dated 29 JUN 1999
>
> In response to your e-mail discussing spotty dry areas on your
> slides, please
> be advised that it appears your slides are drying out prior to being
> coverslipped.
>
> This is avoided by keeping the slides submerged in clearing agent
> prior to
> coverslipping. The H/I RCM (Robot CoverSlipper), all models, employs this
> technology. Slide carriers/baskets are kept in a holding trough
> filled with
> clearing agent until the coverslip is applied.
>
> If you like, please contact us for a demo in your lab to see if
> this resolves
> your problem.
>
>
> Sincerely,
>
>
> Dorothy Murphy
> National Sales Manager
> Hacker Instruments & Industries Inc
>
> Tel: 1-800-4-HACKER or (973) 226-8450
> Fax: (973) 808-8281
> e-mail: HACKERLAB@AOL.COM
>  <A HREF="http://www.hackerinstruments.com/">Hacker Instruments &
> Industries,
> Inc. - Home Page
> </A>
> http://www.hackerinstruments.com
>
>
> ----------------------------------------------------------------------
>
> Date: 8 Jul 1999 09:54:05 -0500
> From: weimer@antibodies-probes.com
> Subject: Re: antibody search
>
> Cayman Chemicals, Ann Arbour, MI 800.364.9897; cayamn@caymanchem.com has
> several antibodies to cyclooxygenase.
>
>
>
> Julie Collins wrote:
> >
> > Does anyone know a source for cyclooxygenase -1 & 2 for FFPE
> human tissue?
> Thanks in advance.
> >
> > Julie Collins
> > Dir, Immunohistochemistry
> > Univ. of Kansas Med. Center
> > Kansas City, KS
> >
>
>
> ----------------------------------------------------------------------
>
> Date: 8 Jul 1999 09:57:27 -0500
> From: dave@biocare.net
> Subject: c-erbB-2 and HercepTest Dilema
>
> I am becoming confused about the FDA approval of Dako's HercepTest.  I
> am hearing all sorts of rumors:
>
> I heard that you don't need to use the Dako detection system for the
> patient to receive treatment.
>
>  I've heard that only 3+ staining validates treatment recommendation.
>
> I've heard that 2+ and 3+ staining validates treatment recommendation.
>
> I've heard that Zymed has a FDA approved c-erbB-2 antibody.  What kind
> of FDA approval,  and can you substitute this antibody instead of using
> Dako polyclonal antibody?
>
> I've heard, if a pathologist does there own in-house study to validate
> their method, they can substitute this procedure and the patient can
> receive treatment; or they can screen with there antibody and validate
> with Dako's HercepTest kit.
>
> I would to get some feedback from some pathologist as well as from
> anybody else who would like to respond.  However, I would like to get at
> the truth.  I would like real data to support what the truth really is.
>
> David Tacha HTL (ASCP)
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 8 Jul 1999 09:58:01 -0500
> From: "R.Wadley" <s9803537@pop3.unsw.edu.au>
> Subject: Re: The future of the Histology lab?
>
> 	Dear Tim,
>
> 	I'm not about to hold my breath waiting for this one.
>
> 	I agree for many routine cases it will be a boon.  But very
> few resin
> impregnation methods are as fast as paraffin or can handle as large a
> specimen.  I can also think of no end of staining procedures that work
> really well on sections that would be impossible on whole tissue samples.
> For every sample that can use this technology, I guess there
> would be be at
> least 1 or 2 that still require the good old fashioned hands on approach.
>
> 	I remember a few years ago all our paraffins were going to be cut
> automatically, collected by robot arm onto slides, stained, cover
> slipped &
> labelled for the pathologist without the need for human
> intervention.  That
> never happened either.
>
> 	While I support further technical advances in the field of
> histology, a
> lot of the proposed new ideas work very well in carefully controlled
> situations & significantly less well in the real world.
>
> 	I'll be ready for the brave new world of histology when
> these grand plans
> actually work, & are reliable & affordable enough to warrant their
> placement in the hospital & pathology labs where most histology scientists
> & technicians work.
>
> 	No, I'm not a Luddite (but my ancestors were transported to
> Tasmania as
> convicts for the crime of machinery breaking).  I do run  a state of the
> art laser analysis facility, & that hasn't stopped good old fashioned
> histology either!
>
> 	Regards
>
> 	Rob.
>
> At 04:22 PM 7/6/99 EDT, you wrote:
> >Histonetters,
> >A recent article in the June 1999 CAP Today (U.S. only),
> "Virtual Tissue,"
> >and the web site www.adpath.com give one possible version of the
> histology
> >lab 5 to 20 years from now (depending on whether you are in a
> research or
> >clinical lab). The article has sample images, the web site only has an
> >explaination of the technology about to hit the histology lab.
> >In short, a piece of pre-stained, resin-embedded tissue is cut into
> >thousands of sections by an automated microtome. Some sections
> are kept for
> >specialized studies but the vast majority are discarded. Instead
> of using
> >the sections, a digital image is made of the block face after
> each section
> >and a 3D digital image of the entire tissue is produced.  Using
> your desktop
> >computer you can then "section" the 'virtual tissue' in the most useful
> >plane for diagnosis.
> >Stains are applied before embedding using fluorescent dyes for
> the common
> >tissue elements. These "stains" can then be interpreted by computer
> >enhancement to produce the "stains" a pathologist is used to seeing.
> >Seems that with DVD technology a microscopical 3D digital image of the
> >entire tissue sample from a case can be stored.
> >If you wonder how it looks, an "H&E" by this method is as good or better
> >than a real H&E. It certainly has better detail.
> >I think the histology lab is about to be 'all shook up.' Hope
> you all are
> >ready!
>
>
>
> R. Wadley, B.App.Sc, M.L.S
> Laboratory Manager
> Cellular Analysis Facility
> School of Microbiology & Immunology
> UNSW, New South Wales, Australia, 2052
> Ph (BH) 	+61 (2) 9385 3517
> Ph (AH)	+61 (2) 9555 1239
> Fax 	+61 (2) 9385 1591
> E-mail	r.wadley@unsw.edu.au
> www	http://www.micro.unsw.edu.au/caf.html
>
>
> ----------------------------------------------------------------------
>
> Date: 8 Jul 1999 09:58:47 -0500
> From: walshsa@webtv.net (sharon walsh)
> Subject: SV40( Large T Antigen)
>
> I am looking for some information on SV40 ( Large T Antigen). The
> application would be formalin-fixed, paraffin-embedded tissue  sections.
> If anyone is using this antibody and could supply me with some
> information, it would be greatly appreciated. I can  be reached during
> the day at (314) 577-8477 or my fax number is (314) 577-8489. Thanks in
> advance for your help.......
>
>
>
> ----------------------------------------------------------------------
>
> Date: 8 Jul 1999 10:00:07 -0500
> From: NTT@shcc.org
> Subject: vendors help
>
> Hi Vendors,
>
> We're budgeting for next year and would like information on available
> cryostats (price, model, availability, etc.)  The hope is that a
> new cryo will
> be bought in the year 2000.  I would appreciate any response with
> information
> and brochures by tomorrow morning.  I'm hoping we get something between
> $15,000
> to $30,000.  SORRY about the limited time.  I just was given this
> request 10
> minutes ago as we missed the deadline yesterday--but got an
> extension till 3pm
> tomorrow.
>
> So if you can respond as soon as possible, I would appreciate it.  You can
> email, fax (503-221-3451), or call me 503-221-1537.
>
> Also, a particular note to Instrumentics (sp).  If you could fax
> me some info
> regarding the CryoJane, that would be great. I'm trying to
> convince my boss to
> get it.
>
> Thank you for all you responses a head of time.
>
> Noi
>
>
> ----------------------------------------------------------------------
>
> Date: 8 Jul 1999 10:00:43 -0500
> From: "Alan Bright" <Bright@dial.pipex.com>
> Subject: Re: Ralph knives
>
> Hi Randy,
>
> I hope this helps.
>
> I used & made this type of Ralf Knife around 20 years ago.
>
> 1. Start off with a 3 X 1 inch slide and diamond score using a small set
> square at the desired position.
>
> 2. Place a wood toothpick on the opposite side of your score line offset
> from the line by say 2mm, this distance is critical and may need
> to be made
> larger or smaller depending on the profile you achieve. Take care and wear
> leather gloves and protective goggles, carefully press down on the slide
> and you should have your Ralf Knife.
>
> 3. To use the knife on a microtome, I placed a standard steel knife in the
> holder with a piece of sheet metal under the steel knife to act as a ledge
> to support the Ralf Knife. Make your Ralf Knife  a few mm. longer than the
> distance from the edge of the knife to the ledge at the base of the knife.
>
> 4. To fix the Ralf Knife  onto the front face of the knife, paraffin wax
> was used by slightly heating both knife and Ralf Knife, and then
> waiting to
> both cooled down.
>
> I hope this information is of assistance to you, please say if you need
> anymore information.
>
>
> Best Regards.
>
> Alan Bright
>
> Bright Instrument Co. Ltd.
> St Margarets Way
> Huntingdon
> PE18 6EB
> England
>
> e-mail: AlanBright@brightinstruments.com
> Tel: +44 (0) 1480 454528
> Fax:+44 (0) 1480 456031
> www.brightinstruments.com
> ++++++++++++++++++++++++++++++++++++++++++++++++++++++=
> - -----Original Message-----
> From: Tindall, Randy D. <TindallR@missouri.edu>
> To: 'microscopy@sparc5.microscopy.com' <microscopy@Sparc5.Microscopy.Com>
> Date: Wednesday, July 07, 1999 12:37
> Subject: Ralph knives
>
>
> >------------------------------------------------------------------------
> >The Microscopy ListServer -- Sponsor: The Microscopy Society of America
> >To  Subscribe/Unsubscribe -- Send Email to ListServer@MSA.Microscopy.Com
> >On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html
> >-----------------------------------------------------------------------.
> >
> >
> >Hi,
> >
> >I'm trying to get some information for a client who wants to try
> making his
> >own glass Ralph (histo) knives.  He says he heard something about a
> >technique for breaking these by hand, without the special
> knifemaker.  Does
> >anyone out there in list-land know anything about this?
> >
> >Thanks much.
> >
> >Randy Tindall
> >Electron Microscope Specialist
> >Electron Microscope Core Facility
> >University of Missouri
> >Columbia, MO  65211
> >
> >
>
>
>
> ----------------------------------------------------------------------
>
> Date: 8 Jul 1999 10:01:14 -0500
> From: Patsy.Ruegg@UCHSC.edu
> Subject: RE: Inexpensive Antibodies
>
> Rick,
> You could not do better than with the University of Iowa Hybridoma Bank.
> They charge $15. Per ml to Universities and $30. Per ml for private
> companies.  Per ml of supernatant.   They are a not for profit
> organization
> funded by the National Institute of Child Health and Human Development.
> They do not have everything, but most.  Contact:
> Karen Jensen
> University of Iowa
> 436 Biology Building
> Iowa, City, IA 52242
> Tel:  319-335-1058
> Fax:  319-335-2077
> e-mail:  dshb@uiowa.edu
>
> 		-----Original Message-----
> 		From:	Richard Maliniak
> [mailto:maliniakrm@worldnet.att.net]
> 		Sent:	Tuesday, July 06, 1999 6:04 PM
> 		To:	HistoNet
> 		Subject:	Inexpensive Antibodies
>
> 		I am currently looking for the lowest possible costs for
> concentrated
> 		antibodies to use on a DAKO autostainer.  I remember a few
> years ago a
> 		company called...Cell Marque {spelling?} had low prices.
> Are they still
> 		around?
>
> 		Rick
>
>
> ----------------------------------------------------------------------
>
> Date: 8 Jul 1999 10:01:52 -0500
> From: "Luty, Carolyn" <cluty@is.mgh.mcgill.ca>
> Subject: CD10
>
> Sorry if this is a repeat question but we are having problems with CD10
> on paraffin sections. Does anyone have any suggestions? We used antigen
> retrieval with citrate buffer but it still did not work. It is a
> Novacastro antibody.
>
>
>
> Carolyn
> Montreal General Hospital
>
>
> ----------------------------------------------------------------------
>
> Date: 8 Jul 1999 10:02:26 -0500
> From: "Saby, Joseph" <Joseph.Saby@wl.com>
> Subject: RE: slide handling
>
> Dear Mr. McCaffrey:
>
> I assume that you got my address from the HistoNet.  Perhaps I am wrong
> about that, and if I am, I apologize for what I am about to write in
> advance.
>
> I participate on the HistoNet to be part of a forum of
> professionals, where
> questions and ideas can be exchanged freely.  There is a place for vendors
> in this forum, a very necessary place I might add.  If a question
> is asked,
> and you have a product which may be a service to the questioner, then you
> are encouraged to reply.  We hope you will reply to HistoNet as a whole so
> we may all learn what you have to say, but you may also reply
> privately.  We
> all hope that under these circumstances that you will
> participate.  Vendors
> are not only a major support for HistoNet and many other
> endeavors, such as
> conventions.  But vendors are on the very cutting edge with their new
> products, and as conscientious professionals we need to know
> about these new
> advances or tools.
>
> But under NO circumstances should any vendor use the address list from
> HistoNet to submit an unsolicited mass mailing.  Again, I encourage you to
> reply to any question asked.  But since I did not ask for your information
> (as seen below), if you obtained my e-mail address from the
> HistoNet, I find
> your conduct unconscionable.  Please refrain from such conduct in the
> future.
>
> Joseph A. Saby, BA HT
> Diagnostic Pathology, Pathology & Experimental Toxicology
> Parke-Davis/Int. Pharm. Res. Div. Warner-Lambert
> Ann Arbor, MI 48105
> Phone: (734) 622-3631
> Fax:     (734) 622-5718
> e-mail:  Joseph.Saby@wl.com
>
>
> - -----Original Message-----
> From: Arthur McCaffrey [mailto:arthur@genex.ltd.uk]
> Sent: Wednesday, July 07, 1999 9:19 AM
> To: sabyj@aa.wl.com
> Subject: slide handling
>
>
> Dear Joseph,
>
> The SlideShow incubation box from Genex Ltd is just what you need to
> semi-automate your immunochemistry protocols. The SlideShow is a
> range of 4
> sizes of humid incubation boxes that have special polymer rails that grip
> your slides or coverslips. There are no clips or fiddly parts,
> simply place
> your slide across 2 rails! Thats it! The rails will prevent all movement
> and allow you to add probes and even wash down slides afterwards.
>
> We have hundreds of customers who use the SlideShow and will not
> go back to
> home-made or glass rail products.
>
> Check out the SlideShow on our web-site
> http://www.genex.ltd.uk/HistoPage.html Prices start at #163#75.00 for a 10
> slide capacity Unit rising to #163#100 for a 30 slide capacity unit.
>
> If you would like more details, e-mail me with your requirements,
> I will be
> happy to help.
>
> Best regards
> Arthur McCaffrey
>
>
>
> ----------------------------------------------------------------------
>
> Date: 8 Jul 1999 10:02:55 -0500
> From: elephantsneedus@erols.com
> Subject: Cytologix
>
> I was curious if anyone has used the Artisan by Cytologix for special
> stains in a clinical setting?  Thanks in advance.
>
> Marian
>
>
>
> ----------------------------------------------------------------------
>
> Date: 8 Jul 1999 10:03:23 -0500
> From: valerie degroff <degroff.1@osu.edu>
> Subject: sequence information
>
> Gayle Callis,
> The National Center of Biotechnology Information has an excellent web
> site for many types of information including the PubMed search engine,
> GenBank sequences, structures etc.  To search for specific nucleotide or
> peptide sequences go to the site and hit the Entrez
> key.                    "www.ncbi.nlm.nih.gov"
>
> Valerie DeGroff
> The Ohio State University
>
>
>
> ----------------------------------------------------------------------
>
> Date: 8 Jul 1999 10:03:56 -0500
> From: mark.lewis@shandon.com
> Subject: RE: shandon/lipshaw rep
>
> Sandy,
>
> I have been off the Histonet for the past 2 weeks and did not see your
> original request.  I would be happy to supply you with information on our
> cryostat line. If you want to contact Sheryl Daniels yourself
> just call toll
> free to 1-800-245-6212 and ask for extension 2013.  Sheryl will
> be more than
> willing to demo a cryostat for you.  Otherwise call the toll free
> number and
> ask for ext. 4013 and I can help you get started.
>
> Thanks !
> Have a superb day !
>
> Mark Lewis
> Technical Specialist
> Shandon
> 1-800-245-6212 ext. 4013
>
>  ----------
> From:  Bonnie.Greer@stjude.org [SMTP:MIME :Bonnie.Greer@stjude.org]
> Sent:  Tuesday, July 06, 1999 5:25 PM
> To:  jennings@mayo.edu; HistoNet@Pathology.swmed.edu
> Subject:  RE: shandon/lipshaw rep
>
> <<File: ENVELOPE.TXT>>
>
> ------------------------------------------------------------------
> --------
> --
>
>
>
>
> > -----Original Message-----
> > From: Anita Jennings [SMTP:jennings@mayo.edu]
> > Sent: Tuesday, July 06, 1999 4:13 AM
> > To: HistoNet@Pathology.swmed.edu
> > Subject: Re: shandon/lipshaw rep
> > > Did you get any replys to your inquiry? We have the best rep
> available in
> > any area. Sheryl Daniels Call the 800 number and leave her a message she
> > is
> > really good at pre and post sale representation. She is truely a sales
> REP
> > not sales person. anita
> > > At 10:38 AM 6/28/99 -0700, you wrote:
> > >I am looking for name and phone for shandon lipshaw rep for Phoenix
> > >metro area.  I'm looking to demo their cryostat.  Or at least receive
> > >info on it.  Any tech with experience with this machine, please respond
> > >also.  Thanks.  Sandy
> > >
> > >
> > > [Greer, Bonnie]  My Shandon rep is DIANA SWAIN DROST and she is
> > excellent. I have the SME cryostat and I love it.......makes lovely
> > sections....easy to operate, to clean etc. >
> >
>
>
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 8 Jul 1999 10:04:24 -0500
> From: Gayle Callis <uvsgc@msu.oscs.montana.edu>
> Subject: inexpensive antibodies message
>
> Help, could someone send that message again, you can do it privately, I
> hit delete by accident before reading.  Bummer!
>
> Gayle Callis
>
>
> ----------------------------------------------------------------------
>
> Date: 8 Jul 1999 10:05:28 -0500
> From: Mbbiocare@aol.com
> Subject: Re: Collagen Type VII and BP Ab's
>
> Back in 1993 I published an abstract in Lab Investigation Vol70#1
> Page 69A
> 1994 "Immunohistochemistry of Type V11 Collagen in The Prostate.  At that
> time we published data on methyl-Carnoys fixative Clone LH7.2 which I had
> gotten from Biodesign Int., Kennebunkport, CA    I did a dilution
> of 1:300
> for 2hrs at RT.
> Later however I did some studies using FFPE tissue using an overnight
> incubation and it worked fine.  I also did some post fixing of
> FFPE slides in
> Carnoy's overnight. I would have to try and find my records to
> give you any
> details. Since you have the antibody from Chemicon, my
> suggestions are to try
> the overnight incubation with primary and use Biocare Medical's detection
> system with their DAB Sparkle, as I had also enhanced my DAB.  Their
> detection system is very sensitive and I have seen slides that are really
> beautiful using their system. Give Vygis Narbutas a call at 800-799-9499
> There is a money back guarantee on all their products.
> Marianne
>
>
> ----------------------------------------------------------------------
>
> Date: 8 Jul 1999 10:06:03 -0500
> From: ross.8@osu.edu (Mary S. Ross)
> Subject: re:antibody search (COX-1,2)
>
> We have successfully used both COX-1 and COX-2 anti-human Ab from Santa
> Cruz on formalin-fixed paraffin-embedded tissue.  (1-800-457-3800)
> (www.scbt.com)
>
> Mary Ross
> Med Micro and Immuno
> Ohio State University
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 8 Jul 1999 10:06:31 -0500
> From: Hedley David Glencross <hedley@hheath.demon.co.uk>
> Subject: muscle bx
>
> Hi everyone
>
> Yet more dialogue re this subject. Once again, I feel I must add my two
> pennies/cents worth. We are committed rollers in talc, and freeze
> directly by immersion in liquid N2. However, we used to freeze the block
> only, and allowed it to come "up" to cutting temperature by storing in
> the cryostat for 15 to 30 minutes. Only then, did we try to orient the
> block on the cryostat chuck, by using OCT and freon. My experience is
> that best results are obtained by freezing only what you want frozen, ie
> muscle. It seems that Jonh Keirnan will see the fruits of this method
> fairly soon, and I can include some histochem as well if he wishes as
> proof. BTW we were able to freeze blocks up to 5 or 6 mm diameter with
> this method.
>
> One last thing, which has just been found out. If ice crystal artefact
> is apparent in the block after freezing, it can often be reduced or even
> remedied by allowing the tissue piece to thaw FULLY and by re freeezing
> properly.
>
> Happy histochemistry everyone.
>
> Regards
> - --
> Hedley David Glencross
>
>
> ----------------------------------------------------------------------
>
> Date: 8 Jul 1999 10:07:01 -0500
> From: Ian Montgomery <ian.montgomery@bio.gla.ac.uk>
> Subject: Clearing agents.
>
> 	Clearing agents for tissue processing in immunohistochemistry.
> 	Xylene is ok but what about the others, chloroform, benzene,
> toluene, amyl acetate etc. I'm a wee bit suspicious about loss of
> antigenicity with chloroform but nothing I'd like to stake the contents of
> my wifes wallet on.
> 	Haven't found a lot in the literature regarding use, any thoughts.
> Ian.
>
>
>
> Dr. Ian Montgomery,
> West Medical Building,
> University of Glasgow,
> Glasgow,
> G12 8QQ,
> Scotland.
> Tel: 0141 339 8855 Extn. 6602.
> Fax: 0141 330 4100.
> e-mail: ian.montgomery@bio.gla.ac.uk
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 8 Jul 1999 10:07:27 -0500
> From: greg tesdall <gtesdall@yahoo.com>
> Subject: Lab floor covering
>
> A small clinic sent me this question. "Do you know if OSHA requires
> that labs have tile rather than carpeting?" I would appreciate some
> Histohelp. Thanks. greg in Nebraska.
> _________________________________________________________
> Do You Yahoo!?
> Get your free @yahoo.com address at http://mail.yahoo.com
>
>
>
> ----------------------------------------------------------------------
>
> Date: 8 Jul 1999 10:08:04 -0500
> From: maliniakrm@worldnet.att.net
> Subject: Declere
>
> Has anyone tried the Cell Marque 'Declere' System that simultaneously
> deparaffinizes, rehydrates and unmasks in 35 minutes?
>  Is the morphology of the tissue good?
>  Are the immunostains crisp?
>
>
> Rick
>
>
> ----------------------------------------------------------------------
>
> Date: 8 Jul 1999 10:08:37 -0500
> From: Wendy Prime <W.Prime@liverpool.ac.uk>
> Subject: Atlas Imprint Smears
>
>
> we are starting to perform imprint smears on tissue
> collected for our Tissue Bank as a means of quickly
> identifying samples  containing tumour cells. As you are
> all aware that a tumour mass can be a very hetrogeneous
> substance.  Can anybody recommend a good atlas for
> identifying different normal and tumour cell types from
> imprint smears or touch preps
>
> thanks in advance
>
>
> Wendy
>
> - ----------------------
> wprime@liverpool.ac.uk
> CTBRC
>
>
>
>
> Here are the messages received yesterday!
>
>
>