RE: CD10

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From:"Hewlett Bryan (CMH)" <HEWLETT@EXCHANGE1.CMH.ON.CA>
To:"'Histonet'" <>, "'Luty, Carolyn'" <>
Date:Sat, 10 Jul 1999 11:39:04 -0400
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What sort of problems?
We use the Novocastra antibody, find it absolutely reliable and robust,
in a variety of fixatives, with or without demineralization. Following
HIER in citrate buffer pH6.0, we incubate AZF or B5 fixed material for 1
hour at RT in a 1:80 dilution of CD10, formaldehyde fixed sections
overnight in a 1:40 dilution. Detection is with LSAB, AEC as chromogen.
What are you using for control material?  



>From: 	Luty, Carolyn[]
>Sent: 	July 8, 1999 2:00 PM
>To: 	Histonet
>Subject: 	CD10
>Sorry if this is a repeat question, but we are having problems with CD10
>on paraffin sections. Does anyone have any suggestions? We used antigen
>retrieval with citrate buffer and it still did not work. It is a
>Novacastro antibody.
>Montreal General Hospital.

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