PFA frozens
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From: | Andrea Kaj <andreakaj@hotmail.com> |
To: | uvsgc@msu.oscs.montana.edu |
Reply-To: | |
Date: | Wed, 07 Jul 1999 00:06:51 PDT |
Content-Type: | text/plain; format=flowed |
Hi Gayle
I work with PFA fixed frozen mouse and rat tissue. What I usually do is
embedding the (wiped off) fixated tissue in OTC, put it in marked plastic
containers and simply put in in the -80 freezer. The tissue can withstand
several freeze-'thaw' cycles, by thawing I mean bringing the tissue to the
cryostat (-22) and back to the freezer (tranported in liquid nitrogen).
Kind regards Andrea
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