PFA frozens

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From:Andrea Kaj <>
Date:Wed, 07 Jul 1999 00:06:51 PDT
Content-Type:text/plain; format=flowed

Hi Gayle
I work with PFA fixed frozen mouse and rat tissue. What I usually do is 
embedding the (wiped off) fixated tissue in OTC, put it in marked plastic 
containers and simply put in in the -80 freezer. The tissue can withstand 
several freeze-'thaw' cycles, by thawing I mean bringing the tissue to the 
cryostat (-22) and back to the freezer (tranported in liquid nitrogen).
Kind regards Andrea

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