Mouse tissue

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From:Andrea Kaj <>
Date:Wed, 07 Jul 1999 00:01:24 PDT
Content-Type:text/plain; format=flowed

Hi Karen
I have worked a lot with frozen mouse tissue, especially intestine from 
foetus. I always use silane coated slides (homemade), and I leave the tissue 
to air dry over night (if it is not fixated, you could fixate it in acetone 
for 5 min. after cutting and then let them air dry). I have the best result 
when fixating the tissue in 4% PFA acouple of hours in the fridge. Hope this 
Kind regards Andrea

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