Help with tissue processing

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From:"Barry, Lilith" <>
To:"'Histonet'" <>
Date:Thu, 15 Jul 1999 16:14:59 -0400
Content-Type:text/plain; charset="iso-8859-1"

Dear colleagues,

I am  a neuroscience technician and have been primarily dealing with
neuronal tissue. Today I was given six jars of pieces of rat organs in
formalin (4% buffered).I have  to paraffin process, section and H&E stain
them for pathological assessment. The organs are heart, trachea, lung,
spleen, kidney, liver and a piece of tibial  muscle. 
Usually I process  the brains in automatic processor programmed 1.5 hours
for each station. Do those specimen need any different times.

Does any of these tissues need special or different handling?

They have been in the fixative since yesterday. I will only be able to
process them on Monday. Should I leave them there until then?

If you have any suggestions no matter how simple they might seem to you, I
am all ears (eyes in this case).

Thanking you in advance,


Lilith Ohannessian-Barry                               
National Research Council                          
Institute of Biological Sciences                  
e-mail; <> 

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