Freezing LARGE muscle specimens
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From: | Margaret Gondo <gondom@valentis.com> |
To: | Histonet@Pathology.swmed.edu |
Reply-To: | |
Date: | Tue, 13 Jul 1999 08:10:44 -0500 |
Content-Type: | text/plain; charset=us-ascii |
Hi All-
In light of all the recent talk (and great tips) on muscle techniques, I
thought I would post this question. I routinely work with large animal
muscles. Currently, I am only freezing down small muscle samples. I would
love to be able to do a cross section of a whole muscle. I'm thinking
along the lines of a pig tibialis or gastroc muscle. At it's widest part,
a pig tibialis (from the animals that we use) is about 3cm by 2 cm. How
would one go about freezing down a section that thick while minimizing
freeze artifact?
Thanks in advance,
Margaret
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