FW: Alexa 430

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From:"Michael J. Lyon, Ph.D." <lyonm@VAX.CS.HSCSYR.EDU>
To:Histonet <HistoNet@Pathology.swmed.edu>
Date:Thu, 15 Jul 1999 14:24:52 -0400
Content-Type:text/plain; charset="iso-8859-1"

I was asked to forward the following:

Michael J. Lyon, Ph.D.
Associate Professor Otolaryngology
315-464-7253 Voice
315-464-5572  FAX

-----Original Message-----
From:	Confocal Microscopy List [mailto:CONFOCAL@LISTSERV.ACSU.BUFFALO.EDU]
On Behalf Of Linda Bookman
Sent:	Monday, July 12, 1999 11:53 AM
Subject:	Alexa 430

Recently, Molecular Probes has stopped producing the LuciferYellow
conjugated to streptavidin. Their substitute product, Alexa 430 was highly
touted and was supposed to be an improvement on the Lucifer.  However, we
are finding on our initial use that not only do we have to use the Alexa at
an incredibly high 1:10 dilution to get any usable signal, but it also fades
almost immediately. We have tried mounting in both Vectashield and glycerine
with no appreciable difference in fade resistance. Does anyone know of
another vendor for the Streptavidin/Lucifer yellow conjugate, and failing
that does anyone have any suggestions for improving the signal on the Alexa
Chris Jones

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