Hi Louise

Not the easiest antibody I have worked with, but Zymed gave excellent
results (cat 13-1700).
I used gastric mucosa as a positive/negative control and the staining
pattern is very distinctive.

There is a Ref: Antigen retrieval solution recommended in Shi SR et al 1996
Biotechnic and Histochem v71:4 190-196.

Do not use picric acid to try to remove formalin pigment as I did, as the
background staining became intense!  The tissue was fixed (a long time ago)
in unbuffered formalin. I have removed formalin pigment with other
antibodies with no problems.

Any other questions, feel free to contact me.
Maree Gould.

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Maree Gould NZCS Dip Sci
Histology Unit Pathology Dept
Medical School
University Of Otago
P.O. Box 913
Dunedin
New Zealand
maree.gould@stonebow.otago.ac.nz
phone: 479 7152  Fax 479 7136

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