E cadherin

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From:Maree Gould <maree.gould@stonebow.otago.ac.nz>
Date:Fri, 2 Jul 1999 09:23:55 +1200
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Hi Louise

Not the easiest antibody I have worked with, but Zymed gave excellent
results (cat 13-1700).
I used gastric mucosa as a positive/negative control and the staining
pattern is very distinctive.

There is a Ref: Antigen retrieval solution recommended in Shi SR et al 1996
Biotechnic and Histochem v71:4 190-196.

Do not use picric acid to try to remove formalin pigment as I did, as the
background staining became intense!  The tissue was fixed (a long time ago)
in unbuffered formalin. I have removed formalin pigment with other
antibodies with no problems.

Any other questions, feel free to contact me.
Maree Gould.


Maree Gould NZCS Dip Sci
Histology Unit Pathology Dept
Medical School
University Of Otago
P.O. Box 913
New Zealand
phone: 479 7152  Fax 479 7136


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