Clearing agents.

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From:Ian Montgomery <ian.montgomery@bio.gla.ac.uk>
To:HistoNet@Pathology.swmed.edu
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Date:Wed, 7 Jul 1999 17:26:19 +0000
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	Clearing agents for tissue processing in immunohistochemistry.
	Xylene is ok but what about the others, chloroform, benzene,
toluene, amyl acetate etc. I'm a wee bit suspicious about loss of
antigenicity with chloroform but nothing I'd like to stake the contents of
my wifes wallet on.
	Haven't found a lot in the literature regarding use, any thoughts.
Ian.



Dr. Ian Montgomery,
West Medical Building,
University of Glasgow,
Glasgow,
G12 8QQ,
Scotland.
Tel: 0141 339 8855 Extn. 6602.
Fax: 0141 330 4100.
e-mail: ian.montgomery@bio.gla.ac.uk





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