Calretinin on cytospins

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From:"Sebree Linda A." <>
To:"'Histonet'" <>
Date:Thu, 15 Jul 1999 14:15:44 -0500

Hi 'netters,

I've been trying to get calretinin (Cell Marque, polyclonal) to work on
acetone-fixed cytospins of normal mesothelial cells and known mesothelioma
cells.  The antibody works beautifully on formalin-fixed, paraffin-embedded
(FFPE) mesotheliomas after HIER but I get zip on the cytology specimens.  Is
this a problem with cell permeabilization?  Would fixation in Zamboni's
fixative or the 3-step formalin/methanol/acetone fixation that works for ER
and PR on frozens work?  Our cytospins are routinely fixed in acetone so I'm
wondering if there is some type of post-fixation step I could employ.

Any help would be greatly appreciated.


Linda Sebree, HT
University of Wisconsin Hospital & Clinics
Department of Laboratory Medicine
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI  53792-2472

Phone:  (608)265-6596
FAX:  (608)263-1568

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