Calretinin on cytospins
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From: | "Sebree Linda A." <la.sebree@hosp.wisc.edu> |
To: | "'Histonet'" <histonet@Pathology.swmed.edu> |
Reply-To: | |
Date: | Thu, 15 Jul 1999 14:15:44 -0500 |
Content-Type: | text/plain |
Hi 'netters,
I've been trying to get calretinin (Cell Marque, polyclonal) to work on
acetone-fixed cytospins of normal mesothelial cells and known mesothelioma
cells. The antibody works beautifully on formalin-fixed, paraffin-embedded
(FFPE) mesotheliomas after HIER but I get zip on the cytology specimens. Is
this a problem with cell permeabilization? Would fixation in Zamboni's
fixative or the 3-step formalin/methanol/acetone fixation that works for ER
and PR on frozens work? Our cytospins are routinely fixed in acetone so I'm
wondering if there is some type of post-fixation step I could employ.
Any help would be greatly appreciated.
Thanks,
Linda
Linda Sebree, HT
University of Wisconsin Hospital & Clinics
Department of Laboratory Medicine
IHC/ISH Laboratory
A4/204-2472
600 Highland Ave.
Madison, WI 53792-2472
Phone: (608)265-6596
FAX: (608)263-1568
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