Attachment of frozens
<< Previous Message | Next Message >>
From: | Andrea Kaj <andreakaj@hotmail.com> |
To: | dennijc@vetmed.auburn.edu, HistoNet@Pathology.swmed.edu |
Reply-To: | |
Date: | Wed, 30 Jun 1999 23:33:42 PDT |
Content-Type: | text/plain; format=flowed |
I don't know if you are talking about fixated frozen sections or raw tissue?
Anyway, I have had good luck letting them dry over night at rt, however
those were fixated in NBF prior to freezing. I have also done it on raw
frozen tissue, - fixated them in acetone for 5 mins. after cutting and
leaving them in the fumehood untill next day. However, the last method has a
worse outcome than the first, why I usually make extra sections. Then again,
brain tissue can be quite troublesome. If your study allows you to do
fixation prior to freezing, you should do it in 10% neutral buffered
formalin or 4% paraformaldehylde (dissolved by cooking in PBS) and leave it
to fix in the fridge for 2-4 hours. Then wipe off excess liquid and freeze
the sections as usual. Hope this helps.
Kind regards Andrea
______________________________________________________
Get Your Private, Free Email at http://www.hotmail.com
<< Previous Message | Next Message >>