Here is my manual protocol for mouses tissues, that
works very well for all mouse tissues (thickness no
more than 3 mm):
1. Isopropanol 70% 30 min
2. Isopropanol 80% 30 min
3. Isopropanol 95% 30 min
4. Isopropanol 99% 30 min
5. Isopropanol:Mineral oil 5:1 50oC 60 min
6. Isopropanol:Mineral oil 2:1 50oC 60 min
7. Mineral oil 1.5 h (or overnight)
8. Paraffin 60oC 30 min
9. Paraffin 60oC 30 min
10. Paraffin 60oC 20 min
11. Paraffin 60oC 20 min.
Advantage of isopropanol consists in that ethanol
have "tide" effect and do tissues hard, but
isopropanol (IPA) not.
Mineral oil = paraffin with low molecular weight
and the infiltration is better and gentle. Mineral
oil is absolutely not toxic for personnel.
Transitions between IPA, MO and paraffin are very
Please let me know about results.
If you need any more details please contact to me.
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