Re: [Histonet] Alcohol fixation and immuno

From:Patti Loykasek

HI Erin. The best situation would be you could speak with this client, and
persuade them to use formalin, too. Let them know your IHC protocols are
optimized for FFPE (probably H&E's would look better, too). If this doesn't
work, obtain some alcohol fixed control  material and optimize your IHC for
this material, too. You will need to try a variety of pretreatments =26
titers.  In essence you will have 2 protocols for your IHC - 1 for FFPE & 1
for alcohol fixed. What fun! Good luck with working all this out.

Patti Loykasek BS, HTL, QIHC
Clinical IHC Supervisor
PhenoPath Laboratories
Seattle, WA

> Hi everyone,
> We occassionally get blocks from outside labs that use alcohol as the
> fixative.  Our pathologist told me that our immunos look pretty bad when we
> handle these cases in the same manner as our routine FFPE cases. He is most
> concerned with Ki67 and K/L.   I tried them with no antigen retrieval but got
> no staining at all.  Any thoughts?  We only do derm.
> Thanks,
> Erin
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