HI Erin. The best situation would be you could speak with this client, and
persuade them to use formalin, too. Let them know your IHC protocols are
optimized for FFPE (probably H&E's would look better, too). If this doesn't
work, obtain some alcohol fixed control material and optimize your IHC for
this material, too. You will need to try a variety of pretreatments =26
titers. In essence you will have 2 protocols for your IHC - 1 for FFPE & 1
for alcohol fixed. What fun! Good luck with working all this out.
Patti Loykasek BS, HTL, QIHC
Clinical IHC Supervisor
> Hi everyone,
> We occassionally get blocks from outside labs that use alcohol as the
> fixative. Our pathologist told me that our immunos look pretty bad when we
> handle these cases in the same manner as our routine FFPE cases. He is most
> concerned with Ki67 and K/L. I tried them with no antigen retrieval but got
> no staining at all. Any thoughts? We only do derm.
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