Use an acetate buffer. It works well for ATPases and acid phosphatases. DO NOT use for esterases (use phosphate)

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318




-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara Albert
Sent: Tuesday, 29 July 2008 9:09 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Sodium barbital buffer substitute


Hi all,
I've been asked to post this question to the list.  What do you use as a substitute for Sodium barbitol buffer in your muscle histochemistries? 
 
Thanks,
Barbara Albert
UCSF Medical Center
San Francisco


      
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