RE: [Histonet] Filter paper

From:"Bonner, Janet"

Watch out that the paper you are using does not change the acidity of your solution (we had a problem with that once using the paper towels!
Janet L. Bonner, HTL (ASCP)
Pathology Laboratory
Florida Hospital Winter Park  


From: on behalf of Wellborn, Janci R
Sent: Fri 7/25/2008 10:37 AM
To: Weems, Joyce;; Gayle Callis;; Mylène de Champlain;
Subject: RE: [Histonet] Filter paper

Both help to keep costs down . . .

-----Original Message-----
From: [] On Behalf Of Weems, Joyce
Sent: Thursday, July 24, 2008 5:45 PM
To:; Gayle Callis;; Mylène de Champlain;
Subject: RE: [Histonet] Filter paper

And paper towels will work when you have run out of filter paper...

-----Original Message-----
From: [] On Behalf Of
Sent: Thursday, July 24, 2008 5:17 PM
To: Gayle Callis;; Mylène de Champlain;
Subject: Re: [Histonet] Filter paper

I sometimes use coffee filters.
------Original Message------
From: Gayle Callis
To: Mylène de Champlain
Subject: Re: [Histonet] Filter paper
Sent: Jul 24, 2008 4:22 PM

What is important is not the number so much as if the paper is high retention and what is filtering speed.  Whatman #4, if I remember correctly, is a medium fast paper, and should be  ideal for filtering staining solution.  Somewhere in the passage of time, I was told not to use paper that was really slow filtering speed and high retention for filtering dye solutions as the paper may retain too much of the dye needed. Now that could be an old wife's tale, but to this day I always use a medium fast paper.

  I also purchase something other than Whatman, as companies sell
equivalents for a much cheaper price.  Whatman 54 should also work.   Big
companies like ThermoScientific give the equivalents for their brand as compared to Whatman papers and are certainly more economical.

Gayle M. Callis

----- Original Message -----
From: "Mylène de Champlain" 
Sent: Wednesday, July 23, 2008 4:31 PM
Subject: [Histonet] Filter paper


  To filter blue celestin, is what I have to use a filter paper number 1 or 4? Thank!

  Mylène de Champlain

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